Effect of interleukin-8 and RANTES on the Gardos channel activity in sickle human red blood cells: role of the Duffy antigen receptor for chemokines
| Autor: | Marie-Dominique Hardy-Dessources, Pierre Couespel du Mesnil, Monique Decastel, Danitza Nebor, Jacques Elion, Marie-Claude Durpès, Danièle Mougenel |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Chemokine Charybdotoxin High density Erythrocytes Abnormal Receptors Cell Surface Anemia Sickle Cell Biology Antigen receptor Humans Interleukin 8 Cell shape Receptor Molecular Biology Cell Shape Chemokine CCL5 Ion Transport Dehydration Interleukin-8 Cell Biology Hematology Intermediate-Conductance Calcium-Activated Potassium Channels Molecular biology Abnormal cytology Oxygen Immunology biology.protein Potassium Molecular Medicine Duffy Blood-Group System |
| Zdroj: | Blood cells, moleculesdiseases. 44(4) |
| ISSN: | 1096-0961 |
| Popis: | We investigated the effects of the chemokines IL-8 and RANTES on the activity of the Gardos channel (GC) of sickle red blood cells (SSRBCs). SSRBCs expressing the Duffy antigen receptor for chemokines (DARC) incubated under oxygenated conditions exhibit GC activation. The deoxygenation-stimulated K(+) loss via the GC is activated by the chemokines in the Duffy-positive SSRBCs. The percentage of cells with high density is 17 times higher in the Duffy-positive group. These findings are consistent with a greater susceptibility of Duffy-positive SSRBCs to inflammatory chemokines leading to GC activation and cellular dehydration and suggest a coupling, promoted by the sickling process, between DARC and the GC. |
| Databáze: | OpenAIRE |
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