Isolation, characterization and HPLC quantification of compounds from Aquilegia fragrans Benth: Their in vitro antibacterial activities against bovine mastitis pathogens
| Autor: | Aiyatullah Shah, Saleem Mushtaq, Md. Niamat Ali, Zeeshan Hamid Shah, Aabid Manzoor Shah, Parvaiz H. Qazi, Mushtaq A. Aga, Faheem Rasool, Hafiz-Ullah Dar, Sajad Ahmad Lone, Aehtesham Hussain, Shabir H. Lone |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aporphines 040301 veterinary sciences Microbial Sensitivity Tests High-performance liquid chromatography 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Column chromatography Drug Discovery medicine Animals Mastitis Bovine Chromatography High Pressure Liquid Benzofurans Pharmacology Traditional medicine Plant Extracts Methanol Aquilegia 04 agricultural and veterinary sciences medicine.disease Sitosterols In vitro Anti-Bacterial Agents Mastitis 030104 developmental biology chemistry Phytochemical Cattle Female Antibacterial activity Magnoflorine |
| Zdroj: | Journal of Ethnopharmacology. 178:9-12 |
| ISSN: | 0378-8741 |
| Popis: | Ethno-pharmacological relevance The underground parts of Aquilegia fragrans are traditionally used for the treatment of wounds and various inflammatory diseases like bovine mastitis. However, there are no reports on the phytochemical characterization and antibacterial studies of A . fragrans. Aim of the study To isolate compounds from the methanol extract of the underground parts of A . fragrans and determine their antibacterial activity against the pathogens of bovine mastitis. The study was undertaken in order to scientifically validate the traditional use of A . fragrans . Materials and methods Five compounds were isolated from the methanol extract of the underground parts of A . fragrans using silica gel column chromatography. Structural elucidation of the isolated compounds was done using spectral data analysis and comparison with literature. High performance liquid chromatography (HPLC) was used for the qualitative and quantitative determination of isolated compounds in the crude methanol extract. The methanol extract and isolated compounds were evaluated for antibacterial activities against mastitis pathogens using broth micro-dilution technique. Results The five isolated compounds were identified as (1) 2, 4-dihydroxyphenylacetic acid methyl ester (2) β-sitosterol (3) Aquilegiolide (4) Glochidionolactone-A and (5) Magnoflorine. A quick and sensitive HPLC method was developed for the first time for qualitative and quantitative determination of four isolated marker compounds from A . fragrans. The crude methanol extract and compound 5 exhibited weak antibacterial activities that varied between the bacterial species (MIC=500–3000 µg/ml). Conclusions The above results show that the crude methanol extract and isolated compounds from A . fragrans exhibit weak antibacterial activities. Further phytochemical and pharmacological studies are required for proper scientific validation of the folk use of this plant species in the treatment of various inflammatory diseases like bovine mastitis. |
| Databáze: | OpenAIRE |
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