Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought

Autor: Georgia Voulgari, Stefanos Kostas, Michail Michailidis, Evangelia Stavridou, Evangelia G. Chronopoulou, Panagiotis Madesis, Irini Nianiou-Obeidat, Nikolaos E. Labrou
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
Thermotolerance
abiotic stress
Hot Temperature
Genetically modified crops
Biology
Genes
Plant
01 natural sciences
Catalysis
Article
Inorganic Chemistry
Transcriptome
lcsh:Chemistry
03 medical and health sciences
transcriptomics
Gene Expression Regulation
Plant
Stress
Physiological
Tobacco
morphophysiology
Physical and Theoretical Chemistry
Molecular Biology
Gene
Transcription factor
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
Abiotic component
N. tabacum
Abiotic stress
Organic Chemistry
fungi
GSTs
food and beverages
General Medicine
Biotic stress
metabolomics
P. vulgaris
Computer Science Applications
Cell biology
primed state
Droughts
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Signal transduction
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 2352, p 2352 (2021)
Volume 22
Issue 5
ISSN: 1422-0067
Popis: Drought and heat stresses are major factors limiting crop growth and productivity, and their effect is more devastating when occurring concurrently. Plant glutathione transferases (GSTs) are differentially expressed in response to different stimuli, conferring tolerance to a wide range of abiotic stresses. GSTs from drought-tolerant Phaseolus vulgaris var. “Plake Megalosperma Prespon” is expected to play an important role in the response mechanisms to combined and single heat and drought stresses. Herein, we examined wild-type N. tabacum plants (cv. Basmas Xanthi) and T1 transgenic lines overexpressing the stress-induced Pvgstu3–3 and Pvgstu2–2 genes. The overexpression of Pvgstu3–3 contributed to potential thermotolerance and greater plant performance under combined stress. Significant alterations in the primary metabolism were observed in the transgenic plants between combined stress and stress-free conditions. Stress-responsive differentially expressed genes (DEGs) and transcription factors (TFs) related to photosynthesis, signal transduction, starch and sucrose metabolism, osmotic adjustment and thermotolerance, were identified under combined stress. In contrast, induction of certain DEGs and TF families under stress-free conditions indicated that transgenic plants were in a primed state. The overexpression of the Pvgstu3–3 is playing a leading role in the production of signaling molecules, induction of specific metabolites and activation of the protective mechanisms for enhanced protection against combined abiotic stresses in tobacco.
Databáze: OpenAIRE
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