Effects of tryptophan-containing peptides on angiotensin-converting enzyme activity and vessel tone ex vivo and in vivo

Autor: Irakli Kopaliani, Sherif Khedr, Andreas Deussen, Birgit Zatschler, Melanie Martin
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Vasodilator Agents
Medicine (miscellaneous)
Angiotensin-Converting Enzyme Inhibitors
In Vitro Techniques
030204 cardiovascular system & hematology
Pharmacology
Umbilical vein
03 medical and health sciences
0302 clinical medicine
In vivo
Rats
Inbred SHR
Internal medicine
medicine.artery
Renin–angiotensin system
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Rats
Wistar
Antihypertensive Agents
Aorta
Cells
Cultured
chemistry.chemical_classification
Nutrition and Dietetics
biology
Protein Stability
Chemistry
Angiotensin-converting enzyme
Dipeptides
Vasodilation
030104 developmental biology
Endocrinology
Enzyme
Dietary Supplements
Hypertension
biology.protein
Female
Vascular Resistance
Endothelium
Vascular
medicine.symptom
Ex vivo
Vasoconstriction
Zdroj: European Journal of Nutrition. 57:907-915
ISSN: 1436-6215
1436-6207
DOI: 10.1007/s00394-016-1374-y
Popis: Over-activation of the renin-angiotensin axis and worsening of vascular function are critical contributors to the development of hypertension. Therefore, inhibition of angiotensin-converting enzyme (ACE), a key factor of the renin-angiotensin axis, is a first line treatment of hypertension. Besides pharmaceutical ACE inhibitors, some natural peptides have been shown to exert ACE-inhibiting properties with antihypertensive effects and potentially beneficial effects on vascular function. In this study, the ACE-inhibiting potential and effects on vascular function of tryptophan-containing peptides were evaluated. The ACE inhibitory action and stability of tryptophan-containing peptides was tested in endothelial cells—a major source of whole body ACE activity. Furthermore, the efficacy of peptides on vascular ACE activity, as well as vessel tone was assessed both ex vivo and in vivo. In human umbilical vein endothelial cells (HUVEC), isoleucine-tryptophan (IW) had the highest ACE inhibitory efficacy, followed by glutamic acid-tryptophan (EW) and tryptophan-leucine (WL). Whereas none of the peptides affected basal vessel tone (rat aorta), angiotensin I-induced vasoconstriction was blocked. IW effectively inhibited aortic ACE activity ex vivo taken from SHRs after 14-weeks of oral treatment with IW. Furthermore, IW treated SHRs showed better endothelium-dependent vessel relaxation compared to placebo. This study shows strong ACE-inhibiting effects of IW, EW and WL in HUVECs and aorta. The peptides effectively counteract angiotensin-induced vasoconstriction and preserve endothelium-dependent vessel relaxation. Thus, tryptophan-containing peptides and particularly IW may serve as innovative food additives with the goal of protection from angiotensin II-induced worsening of vascular function.
Databáze: OpenAIRE
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