Production of 1,5-anhydro-d-fructose by an α-glucosidase belonging to glycoside hydrolase family 31
| Autor: | Atsuo Kimura, Haruhide Mori, Takayoshi Tagami, Hideyuki Matsuura, Janjira Maneesan |
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| Rok vydání: | 2014 |
| Předmět: |
Stereochemistry
Oxocarbenium Oligosaccharides Fructose Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Fungal Proteins Hydrolysis Elimination reaction Glycoside hydrolase family 31 Carbohydrate Conformation Glycoside hydrolase Molecular Biology Carbon Isotopes biology Chemistry α glucosidase Organic Chemistry Aspergillus niger D fructose alpha-Glucosidases General Medicine biology.organism_classification Kinetics Biocatalysis Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 78(12) |
| ISSN: | 1347-6947 |
| Popis: | α-1,4-Glucan lyases [glycoside hydrolase family (GH) 31] catalyze an elimination reaction to form 1,5-anhydro-d-fructose (AF), while GH31 α-glucosidases normally catalyze a hydrolytic reaction. We determined that a small amount of AF was produced by GH31 Aspergillus niger α-glucosidase from maltooligosaccharides by elimination reaction, likely via an oxocarbenium ion intermediate. |
| Databáze: | OpenAIRE |
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