Constructing 3D microtubule networks using holographic optical trapping
| Autor: | Jared Bergman, Olaolu Osunbayo, Michael Vershinin |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0303 health sciences
Multidisciplinary Nanostructure Computer science Holography Environment controlled 02 engineering and technology 021001 nanoscience & nanotechnology Bioinformatics Article law.invention Protein filament 03 medical and health sciences Optical tweezers law Microtubule 0210 nano-technology Biological system Cytoskeleton 030304 developmental biology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep18085 |
| Popis: | Developing abilities to assemble nanoscale structures is a major scientific and engineering challenge. We report a technique which allows precise positioning and manipulation of individual rigid filaments, enabling construction of custom-designed 3D filament networks. This approach uses holographic optical trapping (HOT) for nano-positioning and microtubules (MTs) as network building blocks. MTs are desirable engineering components due to their high aspect ratio, rigidity and their ability to serve as substrate for directed nano-transport, reflecting their roles in the eukaryotic cytoskeleton. The 3D architecture of MT cytoskeleton is a significant component of its function, however experimental tools to study the roles of this geometric complexity in a controlled environment have been lacking. We demonstrate the broad capabilities of our system by building a self-supporting 3D MT-based nanostructure and by conducting a MT-based transport experiment on a dynamically adjustable 3D MT intersection. Our methodology not only will advance studies of cytoskeletal networks (and associated processes such as MT-based transport) but will also likely find use in engineering nanostructures and devices. |
| Databáze: | OpenAIRE |
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