Quantification of In Situ Hybridization Signals in Rat Testes
| Autor: | Koji Takagi, Takako Nomura, Takashi Kogami, Touji Kimura, Jun Kosaka, Teruo Yamada, Junzo Sasaki, Yukari Miki |
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| Rok vydání: | 2004 |
| Předmět: |
Male
0301 basic medicine Messenger RNA Histology 030102 biochemistry & molecular biology Spermiogenesis RNA In situ hybridization Seminiferous Tubules Ribosomal RNA Biology Molecular biology Rats 03 medical and health sciences 030104 developmental biology Meiosis RNA Ribosomal 28S Testis Gene expression Animals Rats Wistar Anatomy Spermatogenesis Metaphase In Situ Hybridization |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 52:813-820 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/jhc.4a6249.2004 |
| Popis: | We performed basic research into quantifying in situ hybridization (ISH) signals in rat testis, a suitable organ for the quantification because germ cells undergo synchronized development and show stage-specific gene expression. In this model experiment, rRNA was selected as the hybridizable RNA in paraffin sections. Specimens fixed with Bouin's fixative and hybridized with digoxygenin-labeled probes could easily be analyzed quantitatively through “posterization” of the images. The amount of rRNA hybridized with the probe was greatest in early primary spermatocytes, followed by pachytene primary spermatocytes, then diplotene spermatocytes, and finally by secondary spermatocytes and spermatids. The amounts reached low levels in metaphase, anaphase, and telophase of meiotic division and early step 1 spermatids, and then slightly increased during spermiogenesis. ISH rRNA staining was a useful parameter for evaluation of the quantitative analysis of mRNA and the levels of hybridizable RNA in tissue sections. (J Histochem Cytochem 52:813–820, 2004) |
| Databáze: | OpenAIRE |
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