Transcriptomic analysis reveals the transcription factors involved in regulating the expression of EPSPS gene, which confers glyphosate resistance of goosegrass (Eleusine indica)
| Autor: | Tai-jie Zhang, Zhang Chun, Chao-jie Yu, Wenlei Guo, Xing-shan Tian |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
EPSPS Agriculture (General) Population Eleusine indica Plant Science 5´-UTR Py-rich stretch element 01 natural sciences Biochemistry Shikimate kinase S1-972 Food Animals Transcriptional regulation education Gene Transcription factor transcription factor education.field_of_study Ecology biology musculoskeletal neural and ocular physiology Wild type transcriptomic 04 agricultural and veterinary sciences biology.organism_classification Molecular biology nervous system Regulatory sequence 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology Agronomy and Crop Science glyphosate resistance 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 20, Iss 8, Pp 2180-2194 (2021) |
| ISSN: | 2095-3119 |
| DOI: | 10.1016/s2095-3119(21)63682-1 |
| Popis: | Glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and overexpression of the EPSPS gene is one of the molecular mechanisms conferring glyphosate resistance in weeds. A regulatory sequence of EPSPS gene was isolated previously, and an alteration in its 5´-untranslated region (UTR) pyrimidine (Py)-rich stretch element is involved in the regulation of EPSPS expression in glyphosate-resistant (GR) Eleusine indica. However, the transcription factors involved in this regulatory sequence remain to be elucidated. In this study, we investigated the regulatory network of EPSPS overexpression associated genes in a GR E. indica population by RNA-seq. The differentially expressed transcript analyses revealed that glyphosate treatment caused an increase in the expression of 2752 unigenes and a decrease in the expression of 4025 unigenes in the GR E. indica, compared to the glyphosate-susceptible (GS) E. indica. Among them, 1373 unigenes were identified to be co-expressed with the EPSPS gene in GR E. indica. GO and KEGG pathway analyses showed that the up-regulated unigenes were mainly enriched in chloroplasts and associated with the shikimate biosynthesis pathway, chlorophy II and peroxisome metabolism processes. Notably, the expression of a Shikimate kinase which catalyzed the conversion of Shikimate to Shikimate 3-phosphate (S3P, a substrate of EPSPS), was also up-regulated. Eight transcription factors were identified as likely to be involved in the regulation of the EPSPS expression, and three of them (ARF2, ARF8 and BPC6) showed more binding sites because of a (CT)n insertion of the 5´-UTR Py-rich stretch element in GR. However, the yeast one-hybrid assay illustrated that ARF8 and BPC6 could bind to the 5´-UTR Py-rich stretch element of wild type EPSPS, but could not bind to the mutated form. Our data suggests that the transcriptional regulation of EPSPS expression is complex and was significantly altered in GR E. indica. These discoveries provide new references for further study of the EPSPS overexpression mechanism that endows glyphosate resistance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect