Radioimmunoassay for the antifreeze polypeptides of the winter flounder: seasonal profile and immunological cross-reactivity with other fish antifreezes
| Autor: | Don Slaughter, Choy L. Hew |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Antigenicity
Radioimmunoassay Cross Reactions medicine.disease_cause Cross-reactivity chemistry.chemical_compound Freezing medicine Animals Bovine serum albumin Chromatography biology Chemistry Fishes General Medicine biology.organism_classification digestive system diseases Biochemistry Sephadex Antifreeze biology.protein Winter flounder Seasons Glutaraldehyde Peptides |
| Zdroj: | Canadian Journal of Biochemistry. 60:824-829 |
| ISSN: | 0008-4018 |
| Popis: | The polymerization of winter flounder antifreeze polypeptide (AFP) with glutaraldehyde to increase its antigenicity and the conjugation of the AFP to bovine serum albumin so that the AFP could carry a radioiodine label have allowed the development of a convenient and reliable radioimmunoassay (RIA) for the antifreeze. A seasonal concentration profile of serum AFP as determined by the RIA corresponded closely with its activity profile. Winter serum concentrations of AFP measured by the RIA (7–11 mg/mL) agreed well with antifreeze recovery from Sephadex G-75 chromatography of serum (6–10 mg/mL), but less so with concentration estimates reported from activity measurements (12–25 mg/mL) (Petzel, D. H., Reisman, H. M. &DeVries, A. L. (1980) J. Exp. Zool. 211, 63–69). A small but measurable immunological cross-reactivity was detected between the antiflounder AFP and other fish antifreeze polypeptides. Such cross-reactivity was quite unexpected considering the large compositional and structural differences among the various AFP and its significance remains unclear. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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