Molecular cloning and characterization of the β-1,3-glucan recognition protein in Anatolica polita
| Autor: | Zhongyuan Liu, Zaixin Li, Xin Xu, Xinfang Mao, Jianhua Yang, Xiaoxia Yang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus beta-Glucans Biology Molecular cloning medicine.disease_cause Microbiology law.invention 03 medical and health sciences 0302 clinical medicine law Lectins Genetics medicine Escherichia coli Animals Amino Acid Sequence Cloning Molecular Gene Peptide sequence Glucans Base Sequence cDNA library General Medicine biology.organism_classification Up-Regulation Coleoptera 030104 developmental biology 030220 oncology & carcinogenesis Recombinant DNA Insect Proteins Carrier Proteins Bacteria Protein Binding |
| Zdroj: | Gene. 697 |
| ISSN: | 1879-0038 |
| Popis: | β-1,3-Glucan recognition protein (βGRP) is an important pattern recognition protein, which could trigger immune response to eliminate pathogens by identifying and combining the pathogenic bacteria. In the present study, a β-1,3-glucan recognition protein gene (ApβGRP) was cloned from a desert beetle Anatolica polita based on the EST sequence of ApβGRP in the suppression subtractive cDNA library. Quantitative real-time PCR (qRT-PCR) results showed that ApβGRP transcript in A. polita was significantly upregulated under the challenge of Escherichia coli and Staphylococcus aureus. Western blot analysis indicated that recombinant ApβGRP expressed in E. coli BL21, has the ability of binding to E. coli and S. aureus. Moreover, agglutination assay suggested that recombinant ApβGRP could agglutinate E. coli, S. aureus and Saccharomyces cerevisiae. The predicted 3D structure showed that ApβGRP possesses a typical β-glucan recognition domain with seven β-strands structures and conserved amino acid sequence. These data indicate that ApβGRP may be involved in immune defense in A. polita and could recognize and bind the bacteria against the invasion of external pathogens. |
| Databáze: | OpenAIRE |
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