Hypoxia preconditioned bone marrow-derived mesenchymal stromal/stem cells enhance myoblast fusion and skeletal muscle regeneration
| Autor: | Bartosz Mierzejewski, Edyta Brzoska, Wladyslawa Streminska, Ilona Kalaszczynska, Joanna Graffstein, Piotr Walczak, Anna M. Różycka, Miroslaw Janowski, Maria A. Ciemerych, Alicja Górzyńska, Iwona Grabowska, Gabriela Muras, Karolina Archacka, Katarzyna Jańczyk-Ilach, Marta Krawczyk |
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| Rok vydání: | 2021 |
| Předmět: |
Myoblast proliferation
Medicine (General) Swine Medicine (miscellaneous) Bone Marrow Cells QD415-436 Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry Myoblasts Myoblast fusion Mice R5-920 Bone Marrow medicine Myocyte Animals Myogenic differentiation Progenitor cell Fusion Hypoxia Muscle Skeletal Migration Cells Cultured Myogenesis Research Stem Cells Mesenchymal stem cell Skeletal muscle Cell Differentiation Mesenchymal Stem Cells Cell Biology BM-MSC Cell biology medicine.anatomical_structure Molecular Medicine Stem cell Normoxia |
| Zdroj: | Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-18 (2021) Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| Popis: | Background The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. The satellite cells remain quiescent and localized between myofiber sarcolemma and basal lamina. They are activated in response to muscle injury, proliferate, differentiate into myoblasts, and recreate myofibers. The stem and progenitor cells support skeletal muscle regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level of oxygen regulates the course of cell proliferation and differentiation. Methods In the present study, we analyzed hypoxia impact on human and pig bone marrow-derived mesenchymal stromal cell (MSC) and mouse myoblast proliferation, differentiation, and fusion. Moreover, the influence of the transplantation of human bone marrow-derived MSCs cultured under hypoxic conditions on skeletal muscle regeneration was studied. Results We showed that bone marrow-derived MSCs increased VEGF expression and improved myogenesis under hypoxic conditions in vitro. Transplantation of hypoxia preconditioned bone marrow-derived MSCs into injured muscles resulted in the improved cell engraftment and formation of new vessels. Conclusions We suggested that SDF-1 and VEGF secreted by hypoxia preconditioned bone marrow-derived MSCs played an essential role in cell engraftment and angiogenesis. Importantly, hypoxia preconditioned bone marrow-derived MSCs more efficiently engrafted injured muscles; however, they did not undergo myogenic differentiation. |
| Databáze: | OpenAIRE |
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