Sequence and expression pattern of the germ line marker vasa in honey bees and stingless bees
| Autor: | E. D. Tanaka, Klaus Hartfelder |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
lcsh:QH426-470
Stingless bee social insect media_common.quotation_subject Insect Developmental Genetics vasa Oogenesis Germline Botany Genetics medicine Molecular Biology Gene media_common biology oogenesis fungi Honey bee biology.organism_classification Oocyte Cell biology lcsh:Genetics medicine.anatomical_structure Apis mellifera Melipona quadrifasciata Research Article |
| Zdroj: | Genetics and Molecular Biology v.32 n.3 2009 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 32, Issue: 3, Pages: 582-593, Published: 19 JUN 2009 Genetics and Molecular Biology, Vol 32, Iss 3, Pp 582-593 (2009) |
| Popis: | Queens and workers of social insects differ in the rates of egg laying. Using genomic information we determined the sequence of vasa, a highly conserved gene specific to the germ line of metazoans, for the honey bee and four stingless bees. The vasa sequence of social bees differed from that of other insects in two motifs. By RT-PCR we confirmed the germ line specificity of Amvasa expression in honey bees. In situ hybridization on ovarioles showed that Amvasa is expressed throughout the germarium, except for the transition zone beneath the terminal filament. A diffuse vasa signal was also seen in terminal filaments suggesting the presence of germ line cells. Oocytes showed elevated levels of Amvasa transcripts in the lower germarium and after follicles became segregated. In previtellogenic follicles, Amvasa transcription was detected in the trophocytes, which appear to supply its mRNA to the growing oocyte. A similar picture was obtained for ovarioles of the stingless bee Melipona quadrifasciata, except that Amvasa expression was higher in the oocytes of previtellogenic follicles. The social bees differ in this respect from Drosophila, the model system for insect oogenesis, suggesting that changes in the sequence and expression pattern of vasa may have occurred during social evolution. |
| Databáze: | OpenAIRE |
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