Mixed infection by Sugarcane mosaic virus and Maize rayado fino virus causing breaking yields in maize in São Paulo state

Autor:	Silvia Regina Galleti, Ivan de Godoy Maia, Gisèle Maria Fantin, Marcos Cesar Gonçalves
Přispěvatelé:	Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal (CPDSV), Universidade Estadual Paulista (Unesp)
Jazyk:	portugalština
Rok vydání:	2007
Předmět:	Dalbulus maidis food.ingredient biology Marafivirus Rhopalosiphum maidis Potyvirus Plant Science biology.organism_classification Horticulture food Plant virus Mixed infection
Zdroj:	Summa Phytopathologica v.33 n.4 2007 Summa phytopathologica Grupo Paulista de Fitopatologia instacron:GPF Summa Phytopathologica, Volume: 33, Issue: 4, Pages: 348-352, Published: DEC 2007 SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP
ISSN:	0100-5405
Popis:	Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:30Z No. of bitstreams: 1 S0100-54052007000400005.pdf: 364277 bytes, checksum: 6475a436e21bac550a5408d4d633cf30 (MD5) Made available in DSpace on 2013-08-22T18:49:30Z (GMT). No. of bitstreams: 1 S0100-54052007000400005.pdf: 364277 bytes, checksum: 6475a436e21bac550a5408d4d633cf30 (MD5) Previous issue date: 2007-12-01 Made available in DSpace on 2013-09-30T19:39:53Z (GMT). No. of bitstreams: 2 S0100-54052007000400005.pdf: 364277 bytes, checksum: 6475a436e21bac550a5408d4d633cf30 (MD5) S0100-54052007000400005.pdf.txt: 25552 bytes, checksum: f69b047056cdf82cdfc12c2a0fb98e65 (MD5) Previous issue date: 2007-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:50:19Z No. of bitstreams: 2 S0100-54052007000400005.pdf: 364277 bytes, checksum: 6475a436e21bac550a5408d4d633cf30 (MD5) S0100-54052007000400005.pdf.txt: 25552 bytes, checksum: f69b047056cdf82cdfc12c2a0fb98e65 (MD5) Made available in DSpace on 2014-05-20T13:50:19Z (GMT). No. of bitstreams: 2 S0100-54052007000400005.pdf: 364277 bytes, checksum: 6475a436e21bac550a5408d4d633cf30 (MD5) S0100-54052007000400005.pdf.txt: 25552 bytes, checksum: f69b047056cdf82cdfc12c2a0fb98e65 (MD5) Previous issue date: 2007-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Os dois principais vírus que infectam o milho no Brasil são o Sugarcane mosaic virus (SCMV) e o Maize rayado fino virus (MRFV), cujos principais vetores são o afídeo Rhopalosiphum maidis e a cigarrinha Dalbulus maidis, respectivamente. O MRFV é freqüentemente encontrado em infecções mistas com fitoplasmas e espiroplasmas, causando as doenças denominadas enfezamentos do milho. em uma lavoura de milho próxima a Santo Antonio da Posse, SP, cercada por campos de cana-de-açúcar, foi encontrada alta incidência de plantas apresentando mosaico, riscas, nanismo e espigas com falhas no enchimento de grãos. Análises serológicas com anti-soros específicos detectaram a presença do SCMV e MRFV nessas plantas. A infecção pelo SCMV também foi confirmada por RT-PCR com primers específicos e análise de seqüências. em observações de preparações contrastadas negativamente em TEM, partículas flexuosas (ca.770 nm) e isométricas (ca.30 nm) foram detectadas. em cortes ultrafinos, inclusões citoplasmáticas, típicas de Potyviridae, foram observadas; não foi encontrada a presença de espiroplasmas nem de fitoplasmas. Esses resultados mostram que a infecção conjunta por SCMV e MRFV pode ser responsável pelos danos encontrados nessa lavoura. The two main viruses infecting maize in Brazil are Sugarcane mosaic virus (SCMV) and Maize rayado fino virus (MRFV), whose main vectors are the aphid Rhopalosiphum maidis and the leafhopper Dalbulus maidis, respectively. MRFV is frequently found in mixed infections with phytoplasms and spiroplasms causing the so-called corn stunting diseases. In a maize commercial field close to Santo Antonio da Posse, São Paulo state, surrounded by sugarcane fields, it was found a high incidence of plants showing mosaic, striping, stunting and cobs with decreased number of grains. Serological analysis with specific antisera detected the presence of SCMV and MRFV in these plants. SCMV infection was also confirmed by RT-PCR with specific primers and sequence analysis. In TEM observations of negatively stained preparations, flexuous (ca.770 nm) and isometric particles (ca.30 nm) were detected. In ultrathin sections, cytoplasmic inclusions, typical of Potyviridae, were observed; neither presence of spiroplasms nor phytoplasms could be noted. These results show that mixed infection by SCMV and MRFV might be responsible for the crop losses. CPDSV Instituto Biológico Universidade Estadual Paulista IB Departamento de Genética Universidade Estadual Paulista IB Departamento de Genética
Databáze:	OpenAIRE
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