Cyanogen bromide fragments of human serum transferrin
| Autor: | Dietmar Grohlich, Anatoly Bezkorovainy |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Alkylation
Cyanogen Acylation Electrophoresis Starch Gel Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound Neuraminic acid Humans Cyanogen Bromide Amino Acids Hexoses chemistry.chemical_classification Molecular mass Transferrin Hexosamines Electrophoresis Disc Molecular biology Amino acid Molecular Weight chemistry Biochemistry Chromatography Gel Neuraminic Acids Cyanogen bromide Ultracentrifuge Peptides Oxidation-Reduction Ultracentrifugation |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure. 310:365-375 |
| ISSN: | 0005-2795 |
| DOI: | 10.1016/0005-2795(73)90118-9 |
| Popis: | Human serum transferrin was fragmented by a combination of methods involving cyanogen bromide, acylation, and reduction-alkylation. Six fragments were isolated in a homogeneous form, and their molecular weights were 26 000; 16 000; 15 000; 9000; 6000; and 4000 (the N-terminal fragment of transferrin), thus accounting for the molecular weight of transferrin (76 000). The carbohydrate present in native transferrin was found in two fragments, that with a molecular weight of 26 000 and that with a molecular weight of 16 000. All amino acids present in native transferrin were accounted for, within experimental error, in the fragments isolated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|