Intravital correlated microscopy reveals differential macrophage and microglial dynamics during resolution of neuroinflammation
| Autor: | Colleen A. Brady, Randall T. Peterson, Ruby D. Kalicharan, Anneke Veenstra-Algra, Nynke Oosterhof, Ben N G Giepmans, Klaas A. Sjollema, Harm H. Kampinga, Jeroen Kuipers, Tjakko J. van Ham |
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| Přispěvatelé: | Molecular Neuroscience and Ageing Research (MOLAR), Center for Liver, Digestive and Metabolic Diseases (CLDM), Clinical Genetics, Neurology |
| Rok vydání: | 2014 |
| Předmět: |
Time Factors
Phagocyte Neutrophils Medicine (miscellaneous) lcsh:Medicine Cell Count 0302 clinical medicine Immunology and Microbiology (miscellaneous) Leukocytes Macrophage Zebrafish IN-VIVO Neurons ELECTRON-MICROSCOPY 0303 health sciences Microscopy Phagocytes Membrane Glycoproteins Microglia Cell Death Microfilament Proteins Brain ALZHEIMERS-DISEASE medicine.anatomical_structure BLOOD MONOCYTES Larva ADULT ZEBRAFISH BRAIN medicine.symptom SPINAL-CORD Intravital microscopy Research Article lcsh:RB1-214 MONOCYTE-DERIVED MACROPHAGES Phagocytosis Green Fluorescent Proteins Neuroscience (miscellaneous) Inflammation Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Immune system Apolipoproteins E medicine lcsh:Pathology Animals APOPTOTIC CELLS Neurodegeneration Neuroinflammation 030304 developmental biology Macrophages lcsh:R CENTRAL-NERVOUS-SYSTEM Astrocytes CHOROID-PLEXUS Immunology 030217 neurology & neurosurgery |
| Zdroj: | Disease Models & Mechanisms, Vol 7, Iss 7, Pp 857-869 (2014) Disease models & mechanisms, 7(7), 857-869. COMPANY OF BIOLOGISTS LTD Disease Models & Mechanisms Disease Models & Mechanisms, 7(7), 857-869. Company of Biologists Ltd |
| ISSN: | 1754-8411 1754-8403 |
| DOI: | 10.1242/dmm.014886 |
| Popis: | Many brain diseases involve activation of resident and peripheral immune cells to clear damaged and dying neurons. Which immune cells respond in what way to cues related to brain disease, however, remains poorly understood. To elucidate these in vivo immunological events in response to brain cell death we used genetically targeted cell ablation in zebrafish. Using intravital microscopy and large-scale electron microscopy, we defined the kinetics and nature of immune responses immediately following injury. Initially, clearance of dead cells occurs by mononuclear phagocytes, including resident microglia and macrophages of peripheral origin, whereas amoeboid microglia are exclusively involved at a later stage. Granulocytes, on the other hand, do not migrate towards the injury. Remarkably, following clearance, phagocyte numbers decrease, partly by phagocyte cell death and subsequent engulfment of phagocyte corpses by microglia. Here, we identify differential temporal involvement of microglia and peripheral macrophages in clearance of dead cells in the brain, revealing the chronological sequence of events in neuroinflammatory resolution. Remarkably, recruited phagocytes undergo cell death and are engulfed by microglia. Because adult zebrafish treated at the larval stage lack signs of pathology, it is likely that this mode of resolving immune responses in brain contributes to full tissue recovery. Therefore, these findings suggest that control of such immune cell behavior could benefit recovery from neuronal damage. |
| Databáze: | OpenAIRE |
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