Murine dendritic cells generated under serum-free conditions have a mature phenotype and efficiently induce primary immune responses
| Autor: | Heide Dierbach, Hendrik Veelken, Marie Follo, Max Warncke, Anna Dodero |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_treatment
T cell Immunology Cell Culture Techniques Biology Immunotherapy Adoptive Culture Media Serum-Free Immunophenotyping Interferon-gamma Mice Immune system Antigen Antigens CD Antigens Neoplasm medicine Immunology and Allergy Animals CD86 MHC class II Mice Inbred BALB C Microscopy Confocal Cell Differentiation Immunotherapy Dendritic cell Dendritic Cells Flow Cytometry Interleukin-12 Peptide Fragments Specific Pathogen-Free Organisms medicine.anatomical_structure Cancer research biology.protein CD80 |
| Zdroj: | Journal of immunological methods. 310(1-2) |
| ISSN: | 0022-1759 |
| Popis: | Vaccination with in vitro-generated dendritic cells (DC) that present tumor-associated antigens is a promising approach for immunotherapy of malignant tumors. For optimization of DC-based vaccination protocols, preclinical tumor models that mimic the clinical situation closely are highly desirable. Strong non-specific T cell activation was observed in experimental immunization of mice with syngeneic DC generated in standard FCS-supplemented culture medium. To avoid deviation of the immune response to FCS-derived antigens, a serum-free culture protocol for in vitro generation of murine DC from bone marrow progenitor cells was developed. In comparison to DC differentiated with FCS supplementation, DC generated under serum-free conditions (sfDC) have a more homogeneous phenotype with higher expression of IL-12 and the differentiation and activation markers CD11c, CD40, CD80, CD83, CD86, DEC-205, and MHC class II. Demonstration of strong uptake of protein and carbohydrate antigens and analysis of the in vivo migration behaviour of sfDC also indicated excellent APC function. Vaccination of mice with peptide-pulsed sfDC efficiently induced an antigen-specific T cell response as assessed by MHC tetramer staining, IFN-gamma ELISPOT and in vivo cytotoxicity assay. sfDC may therefore represent a valuable tool to improve active tumor immunotherapy in animal models. |
| Databáze: | OpenAIRE |
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