Production of haploid-haploid chimeras by sperm injection in the sawfly, Athalia rosae (Hymenoptera)

Autor:	Kugao Oishi, Masatsugu Hatakeyama, Masami Sawa
Rok vydání:	1994
Předmět:	biology fungi Parthenogenesis Oocyte biology.organism_classification Sperm Karyogamy Andrology Chimera (genetics) Sawfly Human fertilization medicine.anatomical_structure Botany Genetics medicine Ploidy Developmental Biology
Zdroj:	Roux's Archives of Developmental Biology. 203:450-453
ISSN:	1432-041X 0930-035X
DOI:	10.1007/bf00188695
Popis:	Mature unfertilized eggs (oocytes) dissected from the ovary of the sawfly Athalia rosae (Hymenoptera) begin parthenogenetic development if exposed to distilled water and produce haploid males. Injection of sperm into mature oocytes through the anterior pole resulted in karyogamy in a fraction of cases which developed as diploid females. No haploid-haploid chimeras due to independent participation of the injected sperm in development were produced. When sperm were injected through the posterior pole, however, fertilization never occurred but haploid-haploid chimeras were produced in a smaller fraction of cases. Both egg nucleus-derived and injected sperm-derived nuclei contributed in forming the germ cells of the chimeric males.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43c3e974ef4f792bd3c5ecafbdbcd9a5 https://doi.org/10.1007/bf00188695 Zobrazit plný text záznamu Full text from SpringerLink