Cocoa consumption reduces NF-κB activation in peripheral blood mononuclear cells in humans

Autor: R. M. Lamuela-Raventos, Emilio Sacanella, Mónica Vázquez-Agell, Esther Tobías, Sandra Camino-López, Gemma Chiva-Blanch, Mireia Urpi-Sarda, Elena Roura, Lina Badimon, Ramon Estruch, Cristina Andres-Lacueva, V. Llorente-Cortés
Rok vydání: 2013
Předmět:
Adult
Male
medicine.medical_specialty
Antioxidant
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Blotting
Western
Anti-Inflammatory Agents
Biological Availability
Vascular Cell Adhesion Molecule-1
Medicine (miscellaneous)
Inflammation
Peripheral blood mononuclear cell
Antioxidants
Beverages
chemistry.chemical_compound
Internal medicine
medicine
Animals
Humans
Prospective Studies
Cacao
Cross-Over Studies
Nutrition and Dietetics
Cell adhesion molecule
business.industry
NF-kappa B
Polyphenols
food and beverages
NF-κB
Middle Aged
Intercellular Adhesion Molecule-1
NFKB1
Crossover study
Pathophysiology
Milk
Endocrinology
chemistry
Immunology
Leukocytes
Mononuclear
Female
medicine.symptom
E-Selectin
Cardiology and Cardiovascular Medicine
business
Cell Adhesion Molecules
Signal Transduction
Transcription Factors
Zdroj: Nutrition, Metabolism and Cardiovascular Diseases. 23:257-263
ISSN: 0939-4753
DOI: 10.1016/j.numecd.2011.03.015
Popis: Epidemiological studies have demonstrated an association between high-polyphenol intake and reduced incidence of atherosclerosis. The healthy effects of cocoa-polyphenols may be due to their antioxidant and anti-inflammatory actions, although the exact mechanisms are unknown and depend on the matrix in which cocoa-polyphenols are delivered. Nuclear factor κB (NF-κB) is a key molecule in the pathophysiology of atherosclerosis involved in the regulation of adhesion molecules(AM) and cytokine expression and its activation is the first step in triggering the inflammatory process. The aim of this study was to evaluate the effect of acute cocoa consumption in different matrices related to the bioavailability of cocoa-polyphenols in NF-κB activation and the expression of AM.Eighteen healthy volunteers randomly received 3 interventions: 40g of cocoa powder with milk (CM), with water (CW), and only milk (M). NF-κB activation in leukocytes and AM (sICAM, sVCAM, E-selectin) were measured before and 6h after each intervention. Consumption of CW significantly decreased NF-κB activation compared to baseline and to CM (P0.05, both), did not change after CM intervention, and significantly increased after M intervention (P = 0.014). sICAM-1 concentrations significantly decreased after 6h of CW and CM interventions (P ≤ 0.026; both) and E-selectin only decreased after CW intervention (P = 0.028). No significant changes were observed in sVCAM-1 concentrations.The anti-inflammatory effect of cocoa intake may depend on the bioavailability of bioactive compounds and may be mediated at least in part by the modulation of NF-κB activation and downstream molecules reinforcing the link between cocoa intake and health.
Databáze: OpenAIRE
Externí odkaz: