Rosmarinic acid potentiates ATRA-induced macrophage differentiation in acute promyelocytic leukemia NB4 cells
| Autor: | Eui-Kyu Noh, Jae-Hoo Park, Jae-Cheol Jo, Dong-Joon Yoon, Hawk Kim, Young Joo Min, SuJin Koh, Sook-Kyoung Heo, Jin Ho Baek |
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| Rok vydání: | 2014 |
| Předmět: |
Acute promyelocytic leukemia
Receptors CCR2 CD14 Retinoic acid Lipopolysaccharide Receptors Receptors CCR1 Tretinoin Biology Peripheral blood mononuclear cell Depsides chemistry.chemical_compound Leukemia Promyelocytic Acute Phagocytosis immune system diseases Differentiation therapy Cell Line Tumor medicine Humans Extracellular Signal-Regulated MAP Kinases neoplasms Pharmacology CD11b Antigen Cell Death Macrophages NF-kappa B Cell Differentiation Drug Synergism medicine.disease Intercellular Adhesion Molecule-1 Enzyme Activation Gene Expression Regulation Neoplastic medicine.anatomical_structure Phenotype chemistry Integrin alpha M Cell culture Cinnamates Immunology Cancer research biology.protein Bone marrow Reactive Oxygen Species Signal Transduction |
| Zdroj: | European journal of pharmacology. 747 |
| ISSN: | 1879-0712 |
| Popis: | Rosmarinic acid (RA, an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid) has a number of biological activities, but little is known about anti-leukemic activities of RA combined with all-trans retinoic acid (ATRA) against acute promyelocytic leukemia (APL) cells. We examined the differentiation marker, CD11b, in bone marrow cells (BMC) of an APL patient, in NB4 cells (APL cell line), and in normal BMC and peripheral blood mononuclear cells (PBMC) of healthy subjects by flow cytometric analysis. ATRA/RA induced expression of CD11b in the BMC of the APL patient and in NB4 cells, but not in normal BMC or PBMC. Therefore, we realized that RA potentiated ATRA-induced macrophage differentiation in APL cells. Further characterization of the induced macrophages showed that they exhibited morphological changes and were able to phagocytose and generate reactive oxygen species. Th also had typical expression of C-C chemokine receptor type 1 (CCR1), CCR2, and intercellular adhesion molecule-1 (ICAM-1). Moreover, the expression of CD11b(+) and CD14(+) cells depended on ERK-NF-κB axis activation. Together, these results indicate that RA potentiates ATRA-induced macrophage differentiation in APL cells. Thus, RA may play an important role as an appurtenant differentiation agent for functional macrophage differentiation in APL. Additionally, the differentiated macrophages might have a normal life span and, they could die. These data indicate that co-treatment with RA and ATRA has potential as an anti-leukemic therapy in APL. |
| Databáze: | OpenAIRE |
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