Biological activity of interleukin-10 in the central nervous system
| Autor: | Cristina Zocchia, Giulio Spiga, Stuart J. Rabin, Italo Mocchetti, Olga N. Chernyshev, Carol A. Colton, John R. Richert, Maria C. Grekova |
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| Rok vydání: | 1997 |
| Předmět: |
Male
STAT3 Transcription Factor medicine.medical_treatment Hypothalamus Inflammation Rats Sprague-Dawley Cellular and Molecular Neuroscience Cricetinae medicine Animals Humans RNA Messenger Phosphorylation STAT3 Neuroinflammation Cells Cultured Microglia biology Cell Biology Cell biology Interleukin-10 Rats DNA-Binding Proteins Glial cell differentiation Interleukin 10 medicine.anatomical_structure Cytokine Immunology biology.protein Trans-Activators Neuroglia Fibroblast Growth Factor 2 medicine.symptom Acute-Phase Proteins |
| Zdroj: | Neurochemistry international. 30(4-5) |
| ISSN: | 0197-0186 |
| Popis: | Cytokines play a crucial role as mediators of inflammation. Astrocytes and microglia are the two major glial cells involved in the central nervous system immune responses. In this study we examined the effects of interleukin-10 (IL-10), one of the naturally occurring inhibitory cytokines, on different types of glial cells in culture such as rat astrocytes, hamster microglia and C6-2B glioma cells. Phosphorylation of signal transducers and activators of transcription (STAT) proteins was used as a marker for IL-10 activity. Within minutes, IL-10 elicited a strong and weak increase in STAT3 and STAT1 phosphorylation, respectively, in human T lymphocytes, suggesting that STAT3 is a main IL-10 signaling event in these cells. In contrast, IL-10 failed to induce STAT3 in glial cells, but elicited a weak increase in STAT1 phosphorylation in microglia and C6-2B glioma cells only, suggesting that in some glial cell population(s) IL-10 may produce cellular responses via activation of the STAT1 pathway. Moreover, in C6-2B cells, IL-10 elicited a decrease in the level of basic fibroblast growth factor mRNA. A similar decrease was observed in adult rat hypothalamus, indicating that this cytokine may regulate glial production of trophic factors. Our data suggest that IL-10 may play a role in glial cell differentiation and proliferation. © 1997 Elsevier Science Ltd. All rights reserved |
| Databáze: | OpenAIRE |
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