Effects of systemic inflammation on insulin sensitivity in horses and inflammatory cytokine expression in adipose tissue
| Autor: | Stephanie E. Reedy, Mandi M. Vick, Barbara A. Murphy, David W. Horohov, R. Frank Cook, Erin L Kennedy, D. R. Sessions, B. P. Fitzgerald |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Time Factors Lipopolysaccharide medicine.medical_treatment Adipose tissue macrophages Adipose tissue White adipose tissue Biology chemistry.chemical_compound Insulin resistance Internal medicine Biopsy medicine Animals Horses Inflammation General Veterinary medicine.diagnostic_test General Medicine medicine.disease Cytokine Endocrinology Adipose Tissue Gene Expression Regulation chemistry Cytokines Female Horse Diseases Tumor necrosis factor alpha Insulin Resistance |
| Zdroj: | American Journal of Veterinary Research. 69:130-139 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.69.1.130 |
| Popis: | Objective—To determine whether an inflammatory challenge induces insulin resistance in horses and examine possible contributions of adipose tissue to inflammatory cytokine production. Animals—15 adult mares. Procedures—Lipopolysaccharide (0.045 μg/kg, IV) or saline solution was administered, and insulin sensitivity was determined by means of the hyperinsulinemic, euglycemic clamp procedure or an adipose tissue biopsy was performed. Adipose tissue samples were collected, and mature adipocytes were obtained. Mature adipocytes were stimulated with lipopolysaccharide or dedifferentiated into preadipocytes and then stimulated with lipopolysaccharide. Interleukin-1, interleukin-6, and tumor necrosis factor A expression in blood, adipose tissue, and adipocytes was quantified with a real-time, reverse transcriptase– PCR assay. Results—Lipopolysaccharide induced a transient increase in insulin sensitivity followed by a reduction in insulin sensitivity at 24 hours. Increased cytokine expression was observed in blood and adipose tissue following administration of lipopolysaccharide, and adipocytes and preadipocytes stimulated with lipopolysaccharide stained positive for tumor necrosis factor A. Expression of interleukin-1, interleukin-6, and tumor necrosis factor A was detected in preadipocytes stimulated with lipopolysaccharide, and interleukin-6 and tumor necrosis factor A were detected in mature adipocytes stimulated with lipopolysaccharide. Conclusions and Clinical Relevance—Results indicated that insulin resistance develops following systemic inflammation in horses and suggested that adipose tissue may contribute to this inflammatory response. Methods to regulate insulin sensitivity may improve clinical outcome in critically ill patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|