Expression of HSP70 in Mytilus californianus following exposure to caffeine
| Autor: | Zoe Rodriguez del Rey, Bradley A. Buckley, Elise F. Granek |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Gills
animal structures Health Toxicology and Mutagenesis Zoology Intertidal zone Management Monitoring Policy and Law Biology Toxicology chemistry.chemical_compound Caffeine Animals Ecotoxicology HSP70 Heat-Shock Proteins Mytilus Ecology Aquatic ecosystem fungi General Medicine Mussel biology.organism_classification Hsp70 chemistry Toxicity Water Pollutants Chemical |
| Zdroj: | Ecotoxicology. 20:855-861 |
| ISSN: | 1573-3017 0963-9292 |
| DOI: | 10.1007/s10646-011-0649-6 |
| Popis: | Caffeine, a biologically active drug with many known molecular targets, is recognized as a contaminant of marine systems. Although the concentrations of caffeine reported from aquatic systems are low (ng/l-μg/l), harmful ecological effects not detected by traditional toxicity tests could occur as a result of caffeine contamination. We used Hsp70, a molecular biomarker of cellular stress, to investigate the sub-lethal cellular toxicity of environmentally relevant concentrations of caffeine on the mussel Mytilus californianus, a dominant species in the rocky intertidal zone along the Oregon Coast. Hsp70 concentrations in the gill and mantle tissue of mussels exposed to 0.05, 0.2, and 0.5 μg/l of caffeine for 10, 20, and 30 days were compared to basal levels in control mussels. Hsp70 in the gill tissue of M. californianus had an initial attenuation of the stress protein followed by a significant up-regulation relative to controls in all but the 0.5 μg/l treatment. Hsp70 in the mantle tissue of mussels exposed to caffeine did not differ from control mussels. This study provides laboratory evidence that environmentally relevant concentrations of caffeine can exert an effect on M. californianus gill tissue at the molecular-level. |
| Databáze: | OpenAIRE |
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