Development of a novel method for amniotic fluid stem cell storage
| Autor: | Giuseppina Comitini, Tullia Maraldi, Anto De Pol, Francesca Beretti, Veronica Barbieri, Giovanni Battista La Sala, Fabrizia Franchi, Laura Bertoni, Manuela Zavatti, Francesca Casciaro |
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| Přispěvatelé: | Zavatti, Manuela, Beretti, Francesca, Casciaro, Francesca, Comitini, Giuseppina, Franchi, Fabrizia, Barbieri, Veronica, Bertoni, Laura, De Pol, Anto, La Sala, Giovanni B., Maraldi, Tullia |
| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Cancer Research Pathology medicine.medical_specialty Amniotic fluid Cellular differentiation Immunology Apoptosis Biology Stem cell marker amniotic fluid stem cell Cryopreservation Specimen Handling Colony-Forming Units Assay Andrology 03 medical and health sciences Freezing medicine Humans Immunology and Allergy Cells Cultured Genetics (clinical) Cell Proliferation Transplantation stem-cell bank Cell growth Stem Cells Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Amniotic stem cells Cell Biology Amniotic Fluid direct freezing 030104 developmental biology Oncology Female Stem cell Biomarkers |
| Zdroj: | Cytotherapy. 19:1002-1012 |
| ISSN: | 1465-3249 |
| DOI: | 10.1016/j.jcyt.2017.04.006 |
| Popis: | Background aims Current procedures for collection of human amniotic fluid stem cells (hAFSCs) indicate that cells cultured in a flask for 2 weeks can then be used for research. However, hAFSCs can be retrieved directly from a small amount of amniotic fluid that can be obtained at the time of diagnostic amniocentesis. The aim of this study was to determine whether direct freezing of amniotic fluid cells is able to maintain or improve the potential of a sub-population of stem cells. Methods We compared the potential of the hAFSCs regarding timing of freezing, cells obtained directly from amniotic fluid aspiration (D samples) and cells cultured in a flask before freezing (C samples). Colony-forming-unit ability, proliferation, morphology, stemness-related marker expression, senescence, apoptosis and differentiation potential of C and D samples were compared. Results hAFSCs isolated from D samples expressed mesenchymal stem cells markers until later passages, had a good proliferation rate and exhibited differentiation capacity similar to hAFSCs of C samples. Interestingly, direct freezing induced a higher concentration of cells positive for pluripotency stem cell markers, without teratoma formation in vivo . Conclusions This study suggests that minimal processing may be adequate for the banking of amniotic fluid cells, avoiding in vitro passages before the storage and exposure to high oxygen concentration, which affect stem cell properties. This technique might be a cost-effective and reasonable approach to the process of Good Manufacturing Process accreditation for stem-cell banks. |
| Databáze: | OpenAIRE |
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