Significant correlation between 14C-5-HT uptake by and 3H-paroxetine binding to platelets from healthy volunteers
| Autor: | Avril Pereira, Bruce Singh, Brian Dean, Kay P. Maguire, Virginia Tuckwell |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Blood Platelets Male Serotonin medicine.medical_specialty Serotonin uptake In Vitro Techniques Tritium Imipramine Radioligand Assay Reference Values Internal medicine medicine Humans Platelet Carbon Radioisotopes Biological Psychiatry Chemistry Middle Aged Paroxetine Kinetics Endocrinology Platelet-rich plasma Antidepressant Female medicine.drug Blood sampling |
| Zdroj: | Biological Psychiatry. 34:356-360 |
| ISSN: | 0006-3223 |
| DOI: | 10.1016/0006-3223(93)90179-h |
| Popis: | The lack of correlation between 5-HT uptake by platelets and the ability of platelet membrane to bind antidepressant drugs, particularly imipramine, has been reported. However, more recently it has been suggested that 3H-paroxetine could be a better drug with which to study the platelet 5-HT uptake mechanism in disease states. We have therefore compared the ability of platelet-rich plasma (PRP) from normal individuals to take up 5-HT with the ability of platelet membranes to bind paroxetine. A significant correlation was apparent between the Vmax of 14C-5-HT uptake by PRP and the Bmax of 3H-paroxetine binding to platelet membrane from 30 individuals (r = 0.6468, p = 0.0001). Furthermore, this correlation was highly significant in the 20 female (r = 0.7768, p = 0.00006) but not in the 10 male volunteers. There was also a significant association between Vmax and Bmax and the month of blood sampling but this did not totally account for the correlation between Vmax and Bmax. The simultaneous measurement of 5-HT uptake by PRP and paroxetine binding to platelet membranes from both depressed patients and matched controls should be carried out to confirm and extend these findings. |
| Databáze: | OpenAIRE |
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