Elevation of long non-coding RNA GAS5 and knockdown of microRNA-21 up-regulate RECK expression to enhance esophageal squamous cell carcinoma cell radio-sensitivity after radiotherapy
| Autor: | E. Li, Wanting Zeng, Jing Lin, Shasha Liao, Zewa Liu, Xiaohua Wu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences Esophageal Neoplasms medicine.medical_treatment Cell Apoptosis Biology GPI-Linked Proteins Radiation Tolerance 01 natural sciences 03 medical and health sciences Cell Line Tumor microRNA Genetics medicine Humans neoplasms Aged 030304 developmental biology 0303 health sciences Gene knockdown Cell growth Middle Aged digestive system diseases Long non-coding RNA Up-Regulation Radiation therapy MicroRNAs medicine.anatomical_structure Cancer research Female RNA Long Noncoding Esophageal Squamous Cell Carcinoma GAS5 010606 plant biology & botany |
| Zdroj: | Genomics. 112:2173-2185 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2019.12.013 |
| Popis: | Objective Lately, lncRNAs have been proposed to function in the radio-sensitivity of tumor cells, yet the role of lncRNA GAS5 in that of esophageal squamous cell carcinoma (ESCC) has scarcely been studied. This study aims to examine GAS5's effects on ESCC cell radio-sensitivity. Methods GAS5, miR-21 and RECK expression in radiation-sensitive and radiation-resistant ESCC tissues, and TE-1 and TE-1-R cells was determined. TE-1 and TE-1-R cells were treated with pcDNA-GAS5 or miR-21 inhibitors to figure out their roles in ESCC cell proliferation, radio-sensitivity, and apoptosis via gain- and loss-of-function experiments. Results We found underexpressed GAS5 and RECK, and overexpressed miR-21 in ESCC. GAS5 elevation and miR-21 inhibition reduced viability and the colony formation ability, and enhanced the apoptosis of ESCC cells under radiation. Conclusion Our study reveals that GAS5 elevation up-regulates RECK expression by down-regulating miR-21 to increase ESCC cell apoptosis after radiation therapy, thus enhancing cell radio-sensitivity. |
| Databáze: | OpenAIRE |
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