Electrical resistance measurements on cerebral capillary endothelial cells — a new technique to study small surface areas
| Autor: | Helmut Franke, Stephan Decker, Hans-Joachim Galla, Martin Erben |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Materials science
Microscope Swine Biophysics Nanotechnology Capillary endothelial cells Cell Communication Arginine Biochemistry Epithelium Tight Junctions law.invention Electrical resistance and conductance law Monolayer Animals Basic amino acids Cells Cultured Tight junction Microcirculation Capillaries Electrophysiology Cerebrovascular Circulation Calcium Endothelium Vascular |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 30:227-238 |
| ISSN: | 0165-022X |
| Popis: | We present a new method which allows resistance measurements in selected cell monolayer areas with a size of less than one mm2. Up to now cell-covered macroscopic filters with areas up to 5 cm2 have been used giving reliable results only in the absence of inhomogeneities or contaminating cells. The new measuring device is posed on the microscope optic to allow an optical characterization of the measuring area and enables us to scan the cell-covered filter. This method has been applied to determine a reversible modulation of the tightness of intercellular contacts between epithelial or endothelial cells in culture. Tight junction resistance is modulated by Ca2+ and basic amino acids in cultured porcine cerebral microvascular endothelial cells that represent the blood-brain barrier. |
| Databáze: | OpenAIRE |
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