Phenotypic and functional characterization of freshly isolated and expanded canine regulatory T cells
| Autor: | Sandra Lange, Anett Sekora, Mathias Freund, Simone Altmann, Anne Knueppel, Christian Junghanss |
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| Rok vydání: | 2011 |
| Předmět: |
Interleukin 2
T-Lymphocytes chemical and pharmacologic phenomena Lymphocyte proliferation Cell Separation Biology Lymphocyte Activation Peripheral blood mononuclear cell T-Lymphocytes Regulatory General Biochemistry Genetics and Molecular Biology Autoimmune Diseases Immune system Dogs immune system diseases hemic and lymphatic diseases Neoplasms medicine Animals IL-2 receptor Cells Cultured Immunity Cellular General Veterinary Interleukin-2 Receptor alpha Subunit Interleukin FOXP3 hemic and immune systems Forkhead Transcription Factors General Medicine Molecular biology In vitro Phenotype Immunology CD4 Antigens Interleukin-2 Animal Science and Zoology medicine.drug |
| Zdroj: | Experimental animals. 60(5) |
| ISSN: | 1881-7122 |
| Popis: | Regulatory T cells (T(reg)) are CD4(+) T lymphocytes with constitutive expression of CD25 and FOXP3, as well as the ability to modulate cellular immune responses. In this study, the phenotypic characteristics, function and feasibility of enrichment and expansion of canine T(reg) were examined. Canine peripheral blood mononuclear cells were isolated and enriched by labelling of CD25, and expansion of T(reg) was achieved by adding interleukin (IL)-2 for 1 week. Phenotypic and functional analyses of T(reg) were performed prior to and after expansion. Canine T(reg) could be phenotypically characterized by CD4, CD25, and FOXP3 expression. Isolation and enrichment of canine T(reg) is possible, but high purities are difficult to achieve without significant cell loss. Expansion of canine T(reg) was possible by adding IL-2 without other growth factors. Higher initial cell numbers seeded allow more substantial T(reg) expansion in vitro. Canine T(reg) have the potential to suppress proliferation of effector T cells (T(eff)). By adding expanded T(reg), a higher capability for suppressing T(eff) could be shown in comparison with freshly isolated T(reg). Enrichment and expansion of canine T(reg) is feasible, and canine T(reg) had similar characteristics to T(reg) from other species. |
| Databáze: | OpenAIRE |
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