Correlation between intestinal BMP2, IFNγ, and neural death in experimental infection with Trypanosoma cruzi

Autor: Simone G. Fonseca, Marcos Vinicius da Silva, Juliana Reis Machado, José Rodrigues do Carmo Neto, Patrícia Resende Alo Nagib, Arthur Wilson Florencio da Costa, Yarlla Loyane Lira Braga, Milton Adriano Pelli de Oliveira, Mara Rúbia Nunes Celes
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Physiology
Quantitative Parasitology
Bone Morphogenetic Protein 2
Parasitemia
Mice
Medical Conditions
Animal Cells
Immune Physiology
Medicine and Health Sciences
Intestinal Mucosa
Immune Response
Myenteric plexus
Protozoans
Trypanosoma Cruzi
Neurons
Innate Immune System
Multidisciplinary
biology
Megacolon
Eukaryota
Interleukin-10
Intestines
Medicine
Cytokines
Anatomy
Cellular Types
Research Article
Chagas disease
Trypanosoma
Colon
Science
Immunology
Myenteric Plexus
Gastroenterology and Hepatology
Bone morphogenetic protein
Bone morphogenetic protein 2
Proinflammatory cytokine
Interferon-gamma
Signs and Symptoms
medicine
Parasitic Diseases
Animals
Chagas Disease
Trypanosoma cruzi
Inflammation
Protozoan Infections
Tumor Necrosis Factor-alpha
Organisms
Biology and Life Sciences
Cell Biology
Molecular Development
medicine.disease
biology.organism_classification
Parasitic Protozoans
Gastrointestinal Tract
Mice
Inbred C57BL
Disease Models
Animal
Cellular Neuroscience
Immune System
Parasitology
Clinical Medicine
Digestive System
Homeostasis
Neuroscience
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 2, p e0246692 (2021)
ISSN: 1932-6203
Popis: Megacolon is one of the main late complications of Chagas disease, affecting approximately 10% of symptomatic patients. However, studies are needed to understand the mechanisms involved in the progression of this condition. During infection by Trypanosoma cruzi (T. cruzi), an inflammatory profile sets in that is involved in neural death, and this destruction is known to be essential for megacolon progression. One of the proteins related to the maintenance of intestinal neurons is the type 2 bone morphogenetic protein (BMP2). Intestinal BMP2 homeostasis is directly involved in the maintenance of organ function. Thus, the aim of this study was to correlate the production of intestinal BMP2 with immunopathological changes in C57Bl/6 mice infected with the T. cruzi Y strain in the acute and chronic phases. The mice were infected with 1000 blood trypomastigote forms. After euthanasia, the colon was collected, divided into two fragments, and a half was used for histological analysis and the other half for BMP2, IFNγ, TNF-α, and IL-10 quantification. The infection induced increased intestinal IFNγ and BMP2 production during the acute phase as well as an increase in the inflammatory infiltrate. In contrast, a decreased number of neurons in the myenteric plexus were observed during this phase. Collagen deposition increased gradually throughout the infection, as demonstrated in the chronic phase. Additionally, a BMP2 increase during the acute phase was positively correlated with intestinal IFNγ. In the same analyzed period, BMP2 and IFNγ showed negative correlations with the number of neurons in the myenteric plexus. As the first report of BMP2 alteration after infection by T. cruzi, we suggest that this imbalance is not only related to neuronal damage but may also represent a new route for maintaining the intestinal proinflammatory profile during the acute phase.
Databáze: OpenAIRE
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