Specific protease activity indicates the degree of Pseudomonas aeruginosa infection in chronic infected wounds
| Autor: | Philip Stephens, Katja E. Hill, Robert G. Price, David Wynne Williams, Dirk Wildeboer, Andrew David Riddell, Fiona Jeganathan, Ramadan A. Abuknesha, David Thomas, Patricia Elaine Price |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Microbiology (medical) Chronic wound medicine.medical_specialty Adolescent medicine.medical_treatment Peptide Biology medicine.disease_cause Microbiology Young Adult Antibiotic resistance Medical microbiology medicine Humans Pseudomonas Infections Child Aged Aged 80 and over chemistry.chemical_classification Protease Pseudomonas aeruginosa Infant General Medicine Middle Aged biology.organism_classification Bacterial Load Enzyme assay Infectious Diseases chemistry Child Preschool Chronic Disease Wound Infection biology.protein Female medicine.symptom Bacteria Peptide Hydrolases |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 31:2183-2189 |
| ISSN: | 1435-4373 0934-9723 |
| Popis: | Chronic non-healing wounds are a major health problem with resident bacteria strongly implicated in their impaired healing. A rapid-screen to provide detailed knowledge of wound bacterial populations would therefore be of value and help prevent unnecessary and indiscriminate use of antibiotics-a process associated with promoting antibiotic resistance. We analysed chronic wound fluid samples, which had been assessed for microbial content, using 20 different fluorescent labelled peptide substrates to determine whether protease activity correlated with the bacterial load. Eight of the peptide substrates showed significant release of fluorescence after reaction with some of the wound samples. Comparison of wound fluid protease activities with the microbiological data indicated that there was no correlation between bacterial counts and enzyme activity for most of the substrates tested. However, two of the peptide substrates produced a signal corresponding with the microbial data revealing a strong positive correlation with Pseudomonas aeruginosa numbers. This demonstrated that short fluorescent labelled peptides can be used to detect protease activity in chronic wound fluid samples. The finding that two peptides were specific indicators for the presence of P. aeruginosa may be the basis for a diagnostic test to determine wound colonisation by this organism. |
| Databáze: | OpenAIRE |
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