Mepacrine impairs neutrophil response after acute lung injury in rats. Effects on neutrophil migration
| Autor: | Lydia L. Polomski, J. Bernard L. Gee, Patricia S. Mikes |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lung Diseases Male Neutrophils Neutrophile Mepacrine Inflammation Lung injury Biology Pharmacology Phospholipase A2 Cell Movement medicine Animals Leukocytosis Mast Cells Chemotactic Factors Interleukin-8 Mast cell Silicon Dioxide Rats Inbred F344 Rats Trachea medicine.anatomical_structure Instillation Drug Quinacrine Immunology Acute Disease Alveolar macrophage biology.protein medicine.symptom medicine.drug |
| Zdroj: | The American review of respiratory disease. 138(6) |
| ISSN: | 0003-0805 |
| Popis: | Intraalveolar leukocytosis is integral in initiating and perpetuating airspace inflammatory reactions. We used intratracheal instillation of silica suspensions in adult male rats to cause neutrophil flux (32% increase over saline controls) without creating a protein leak, so simulating an early inflammatory response. We examined the in vivo effects of a known phospholipase A2 inhibitor (mepacrine) and the two mast cell active agents (cyproheptadine and reserpine) on lung lavage fluid chemotactic capability, alveolar macrophage (AM) production of chemotactic factor(s), and neutrophil diapedesis. Only mepacrine significantly depressed the leukocytosis (from 32% to 8% of total cells), with a similar diminution in AM chemotaxin production. Separate in vitro experiments using mepacrine-pretreated neutrophils and macrophages gave evidence that mepacrine: (1) diminishes neutrophil response to chemotaxin(s), (2) inhibits spontaneous, random neutrophil movement, and (3) diminishes macrophage-derived chemotactic factor production. These observations suggest that the earliest events in alveolar inflammatory reactions probably involve local production of chemotactic factors by AM, and that mepacrine's anti-inflammatory action results from inhibitory influences on both macrophage and neutrophil populations. |
| Databáze: | OpenAIRE |
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