Histopathological and immunohistochemical studies of membranes of deacetylated chitin derivatives implanted over rat calvaria
| Autor: | Michio Ito, A. H. Kafrawy, Yuichi Hidaka, Koji Mori, Hiroshi Yagasaki |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Materials science Biocompatibility Biomedical Engineering Synthetic membrane Chitin Inflammation Calvaria macromolecular substances Rats Sprague-Dawley Biomaterials Chitosan chemistry.chemical_compound medicine Animals Skull Granulation tissue Acetylation Membranes Artificial Prostheses and Implants Anatomy Immunohistochemistry Molecular biology Rats carbohydrates (lipids) medicine.anatomical_structure Membrane chemistry medicine.symptom |
| Zdroj: | Journal of Biomedical Materials Research. 46:418-423 |
| ISSN: | 1097-4636 0021-9304 |
| Popis: | Membranes made of 65, 70, 80, 94, and 100% deacetylated chitin (chitosan) were implanted subperiosteally over the calvaria of 100 rats. Reactions were studied at 1, 2, 4, and 8 weeks after implantation. Membranes prepared with 65, 70, and 80% deacetylated chitin initially elicited marked inflammatory reactions that subsided in time with granulation tissue formation and osteogenesis. Osteocalcin-positive cells were detected immunohistochemically in the granulation tissue. On the other hand, membranes made of 94% deacetylated chitin and chitosan showed mild inflammation and minimal osteogenesis. The results indicate that membranes made of 65, 70, and 80% deacetylated chitin enhance osteogenesis at the site of their implantation. However, the initially severe inflammatory reaction associated with these materials needs to be controlled before the materials would be suitable for clinical application. |
| Databáze: | OpenAIRE |
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