Comparative plasma proteomic studies of pulmonary TiO2 nanoparticle exposure in rats using liquid chromatography tandem mass spectrometry
| Autor: | Jinghai Yi, Megan M. Maurer, Stephen J. Valentine, Timothy R. Nurkiewicz, Gregory C. Donohoe, Hossein Maleki, Carroll R. McBride |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Proteomics 0301 basic medicine Proteome Biophysics Metal Nanoparticles Tandem mass spectrometry medicine.disease_cause Biochemistry Article Rats Sprague-Dawley 03 medical and health sciences Tandem Mass Spectrometry Liquid chromatography–mass spectrometry medicine Animals Blood Coagulation Lung Titanium Inhalation exposure Inhalation Exposure Principal Component Analysis Chromatography Chemistry 030111 toxicology Blood Proteins Blood proteins Rats Liver Signal transduction Reactive Oxygen Species Oxidative stress Chromatography Liquid Signal Transduction |
| Zdroj: | Journal of Proteomics. 130:85-93 |
| ISSN: | 1874-3919 |
| Popis: | Mounting evidence suggests that pulmonary exposure to nanoparticles (NPs) has a toxic effect on biological systems. A number of studies have shown that exposure to NPs result in systemic inflammatory response, oxidative stress, and leukocyte adhesion. However, significant knowledge gaps exist for understanding the key molecular mechanisms responsible for altered microvasculature function. Utilizing comprehensive LC-MS/MS and comparative proteomic analysis strategies, important proteins related to TiO2 NP exposure in rat plasma have been identified. Molecular pathway analysis of these proteins revealed 13 canonical pathways as being significant (p ≤ 0.05), but none were found to be significantly up or down regulated (z > ∣2∣). This work lays the foundation for future research that will monitor relative changes in protein abundance in plasma and tissue as a function of post-exposure time and TiO2 NP dosage to further elucidate mechanisms of pathway activation as well as to decipher other affected pathways. |
| Databáze: | OpenAIRE |
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