Determination of valproic acid and its six metabolites in human serum by LC-MS/MS and application to interaction with carbapenems in epileptic patients

Autor:	Xuping Yang, Zhenwei Jiang, Yan Jiang, Jing Ling, Lulu Dong, Sulan Zou, Rong Chen, Nan Hu
Rok vydání:	2022
Předmět:	Pharmacology Clinical Biochemistry Drug Discovery General Medicine Molecular Biology Biochemistry Analytical Chemistry
Zdroj:	Biomedical chromatography : BMC.
ISSN:	1099-0801
Popis:	Valproic acid (VPA) is a classic medication for several types of epilepsy and mood disorders, and some of its effectiveness and toxicity were associated with metabolites. Although many reports have reported the drug-drug interactions of VPA, no study has focused on the influence of carbapenems (CBPMs) on VPA's active metabolites. An LC-MS/MS method for determining VPA and its six metabolites (3-OH-VPA, 4-OH-VPA, 2-ene-VPA, 4-ene-VPA, 3-keto-VPA and 2-PGA) in human serum was established and applied to evaluate drug-drug interaction with CBPMs in epileptic patients. The stable isotope VPA-d6 was used as an internal standard. Analytes in serum samples (50 μL) were isolated using a Kinetex C
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43ad44cd7211baabd185a54e5798561e https://pubmed.ncbi.nlm.nih.gov/36520520 Zobrazit plný text záznamu Plný text