Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes
| Autor: | Masamichi Isogai, Akihiro Tamura, Tomokazu Sugai, Yukari Takahashi, Aki Igarashi, Hideki Takahashi, Nobuyuki Yoshikawa, Hajime Yaegashi, Noriko Yamagishi, Tsubasa Takahashi, Kousuke Yamagata, Emiko Sugawara |
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| Rok vydání: | 2009 |
| Předmět: |
Phytoene desaturase
Arabidopsis thaliana Cucurbit Genetic Vectors Arabidopsis Virus-induced gene silencing Lyases Gene Knockout Techniques Solanum lycopersicum Gene Expression Regulation Plant Virology Tobacco Botany RNA Viruses Gene silencing Gene Silencing Vector (molecular biology) Gene Genetics biology fungi food and beverages Fabaceae Apple latent spherical virus vector Meristem biology.organism_classification Phenotype Legume Cucurbitaceae Magnesium chelatase RNA Plant Genetic Engineering Oxidoreductases |
| Zdroj: | Virology. 386:407-416 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2009.01.039 |
| Popis: | Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis thaliana, and economically important crops such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors could be successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCY1 gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species. |
| Databáze: | OpenAIRE |
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