Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
| Autor: | Jeanne F. Loring, Jonathan M. Auerbach, Timo Otonkoski, Ivan Damjanov, Karin Astrid Maria Gertow, Olga Epifano, Stefan Przyborski, Lars Ährlund-Richter |
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| Rok vydání: | 2007 |
| Předmět: |
Genetic Markers
Male Pluripotent Stem Cells endocrine system Mesoderm Embryonal Carcinoma Stem Cells endocrine system diseases Injections Subcutaneous Transplantation Heterologous Ectoderm Cell Separation Biology Kidney Mice 03 medical and health sciences 0302 clinical medicine Testis medicine Animals Humans Kidney surgery Embryonic Stem Cells Tumor Stem Cell Assay 030304 developmental biology 0303 health sciences Histocytological Preparation Techniques Cell Biology General Medicine Anatomy medicine.disease Embryonic stem cell Cell biology Transplantation medicine.anatomical_structure embryonic structures Teratoma Endoderm Stem cell 030217 neurology & neurosurgery Stem Cell Transplantation Developmental Biology |
| Zdroj: | Current Protocols in Stem Cell Biology |
| ISSN: | 1938-8969 1941-7322 |
| Popis: | This unit describes protocols on how to assess the developmental potency of human embryonic stem cells (hESCs) by performing xenografting into immunodeficient mice to induce teratoma formation. hESCs can be injected under the testis capsule, or alternatively into the kidney or subcutaneously. Teratomas that develop from grafted hESCs are surgically removed, fixed in formaldehyde, and paraffin embedded. The tissues in the teratoma are analyzed histologically to determine whether the hESCs are pluripotent and form tissues derived from of all three embryonic germ layers (ectoderm, mesoderm, and endoderm). Teratomas can also be fixed in Bouin's or cryosectioned for analysis, and they can be analyzed by immunohistochemistry for tissue markers. Methods for these procedures are included in this unit. |
| Databáze: | OpenAIRE |
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