Transmission of tuberculosis in Havana, Cuba: a molecular epidemiological study by IS6110 restriction fragment length polymorphism typing
| Autor: | Raúl Díaz, José A Valdivia, J Sevy-Court, E Restrepo, R.I. Gómez, R Rumbaut, D. van Soolingen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Adult DNA Bacterial Male medicine.medical_specialty Tuberculosis IS6110 RFLP Havana lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 DNA fingerprinting lcsh:QR1-502 lcsh:Microbiology Disease Outbreaks Mycobacterium tuberculosis Epidemiology medicine Humans Typing Aged Genetics biology Transmission (medicine) Incidence transmission Outbreak Cuba Middle Aged medicine.disease biology.organism_classification Virology DNA profiling Female epidemiology Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 96, Iss 4, Pp 437-443 (2001) Memórias do Instituto Oswaldo Cruz, Volume: 96, Issue: 4, Pages: 437-443, Published: MAY 2001 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | The combination of molecular and conventional epidemiological methods has improved the knowledge about the transmission of tuberculosis in urban populations. To examine transmission of tuberculosis in Havana, Cuba, with DNA fingerprinting, we studied 51 out of 92 Mycobacterium tuberculosis strains isolated from tuberculosis patients who resided in Havana and whose infection was culture-confirmed in the period from September 1997 to March 1998. Isolates from 28 patients (55%) had unique IS6110 restriction fragment length polymorphism (RFLP) patterns, while isolates from 23 others (45%) had identical patterns and belonged to 7 clusters. Three clusters consisting of six, five and two cases were each related to small outbreaks that occurred in a closed setting. Three other clustered cases were linked to a large outbreak that occurred in another institution. Younger patients were more correlated to clustering than older ones. The finding that 45% of the isolates had clustered RFLP patterns suggests that recent transmission is a key factor in the tuberculosis cases in Havana. The IS6110 RFLP typing made it possible to define the occurrence of outbreaks in two closed institutions. |
| Databáze: | OpenAIRE |
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