Distribution and chemical composition of estrogen receptor β neurons in the paraventricular nucleus of the female and male mouse hypothalamus

Autor: Maranda K. Thompson, Aaron Z. Handa, Robert J. Handa, Mario G. Oyola
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
endocrine system
medicine.medical_specialty
Vasopressin
Tyrosine 3-Monooxygenase
Corticotropin-Releasing Hormone
Green Fluorescent Proteins
Estrogen receptor
Neuropeptide
Mice
Transgenic
Biology
Oxytocin
Article
Mice
03 medical and health sciences
Corticotropin-releasing hormone
0302 clinical medicine
Internal medicine
medicine
Animals
Estrogen Receptor beta
Estrogen receptor beta
Neurons
Analysis of Variance
Sex Characteristics
General Neuroscience
digestive
oral
and skin physiology
Estrogen Receptor alpha
Colocalization
Arginine Vasopressin
Mice
Inbred C57BL
030104 developmental biology
Endocrinology
Gene Expression Regulation
nervous system
Hypothalamus
Female
hormones
hormone substitutes
and hormone antagonists
030217 neurology & neurosurgery
Paraventricular Hypothalamic Nucleus
medicine.drug
Zdroj: Journal of Comparative Neurology. 525:3666-3682
ISSN: 0021-9967
Popis: Activation of estrogen receptor beta (ERβ)-expressing neurons regulates the mammalian stress response via the hypothalamic-pituitary-adrenal (HPA) axis. These neurons densely populate the paraventricular nucleus of the hypothalamus (PVN). Recent research has revealed striking differences between rat and mouse PVN cytochemistry, but careful exploration of PVN ERβ neurons in mice has been hindered by a lack of specific ERβ antisera. Therefore, we used male and female transgenic mice expressing EGFP under the control of the mouse ERβ promoter (ERβ-EGFP) to examine the chemical architecture of PVN ERβ cells. Using immunohistochemistry, we found that 90% of ERβ-immunoreactivity (-ir) colocalized with EGFP. Cellular colocalization of EGFP with neuropeptides, transcription modulators, and neuronal tracers was examined throughout the PVN. ERβ-EGFP cells expressed oxytocin more abundantly in the rostral (71±3%) than caudal (33±8%) PVN. Arginine vasopressin colocalized with EGFP more often in females (18±3%) than males (4±1%). Moreover, estrogen receptor α-ir colocalized with ERβ-EGFP at low levels (15±3%). Using a corticotropin releasing hormone (CRH)-cre driver X tdTomato reporter mouse, we found a moderate colocalization with ERβ-ir (48±16%) in the middle PVN. Peripheral injection of fluorogold revealed that the rostral PVN ERβ-EGFP cells are neuroendocrine neurons whereas non-neuroendocrine (presumably pre-autonomic) ERβ-EGFP neurons predominated in the posterior PVN. These data demonstrate chemoarchitectural differences in ERβ neurons of the mouse PVN that are different from that previously described for the rat, thus, elucidating potential neuronal pathways involved in the regulation of the HPA axis in mice. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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