Proteína capsidial e RNA do Cole latent virus não estão presentes dentro dos cloroplastos de células infetadas de Chenopodium quinoa

Autor:	P. Belintani, José O. Gaspar
Přispěvatelé:	Universidade Estadual Paulista (Unesp)
Rok vydání:	2003
Předmět:	Carlavirus animal structures electron microscopy Plant virus Brassica Plant Science Biology biology.organism_classification Ribosome Chenopodium quinoa Molecular biology Virus Chloroplast Organelle Ultrastructure virus replication
Zdroj:	SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Fitopatologia Brasileira v.28 n.1 2003 Fitopatologia Brasileira Sociedade Brasileira de Fitopatologia (SBF) instacron:SBF
ISSN:	0100-4158
DOI:	10.1590/s0100-41582003000100012
Popis:	Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:48:59Z No. of bitstreams: 1 S0100-41582003000100012.pdf: 1233224 bytes, checksum: 025dc3a8bf897075d4bbff27c2f37746 (MD5) Made available in DSpace on 2013-08-22T18:48:59Z (GMT). No. of bitstreams: 1 S0100-41582003000100012.pdf: 1233224 bytes, checksum: 025dc3a8bf897075d4bbff27c2f37746 (MD5) Previous issue date: 2003-01-01 Made available in DSpace on 2013-09-30T18:45:26Z (GMT). No. of bitstreams: 2 S0100-41582003000100012.pdf: 1233224 bytes, checksum: 025dc3a8bf897075d4bbff27c2f37746 (MD5) S0100-41582003000100012.pdf.txt: 21675 bytes, checksum: 29869b279de8019a5c9862ec683b20da (MD5) Previous issue date: 2003-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:03Z No. of bitstreams: 2 S0100-41582003000100012.pdf: 1233224 bytes, checksum: 025dc3a8bf897075d4bbff27c2f37746 (MD5) S0100-41582003000100012.pdf.txt: 21675 bytes, checksum: 29869b279de8019a5c9862ec683b20da (MD5) Made available in DSpace on 2014-05-20T14:04:03Z (GMT). No. of bitstreams: 2 S0100-41582003000100012.pdf: 1233224 bytes, checksum: 025dc3a8bf897075d4bbff27c2f37746 (MD5) S0100-41582003000100012.pdf.txt: 21675 bytes, checksum: 29869b279de8019a5c9862ec683b20da (MD5) Previous issue date: 2003-01-01 O vírus latente da couve (Cole latent virus, CoLV), gênero Carlavirus, foi estudado, por microscopia eletrônica de transmissão e técnicas bioquímicas, em relação à ultra-estrutura das células infetadas de Chenopodium quinoa, e de sua associação com os cloroplastos. O CoLV foi observado como partículas dispersas pelo citoplasma entremeadas com vesículas membranosas e ribossomos e/ou como densas massas de partículas. Estes partículas reagiram por imunomarcação com anti-soro policlonal para o CoLV. Morfologicamente, cloroplastos, mitocôndrias e núcleos mostraram-se inalterados e partículas virais não foram encontradas dentro dessas organelas. Entretanto, agregados de partículas virais foram freqüentemente vistos em associação com a membrana externa dos cloroplastos e ocasionalmente com peroxissomos. Cloroplastos foram purificados em gradiente de Percoll e as proteínas e os RNA foram extraídos e analisados, respectivamente, por Western blot e Northern blot. Proteína capsidial e RNA associados ao CoLV não foram detectados nessa organela. Os resultados aqui obtidos indicam que a associação CoLV/cloroplastos, observada nos estudos de microscopia eletrônica, é possivelmente um evento casual dentro da célula hospedeira e que o vírus não se multiplica dentro dessa organela. Cole latent virus (CoLV), genus Carlavirus, was studied by electron microscopy and biochemical approaches with respect both to the ultrastructure of the Chenopodium quinoa infected cells and to its association with chloroplasts. The CoLV was observed to be present as scattered particles interspersed with membranous vesicles and ribosomes or as dense masses of virus particles. These virus particles reacted by immunolabelling with a polyclonal antibody to CoLV. Morphologically, chloroplasts, mitochondria and nuclei appeared to be unaltered by virus infection and virus particles were not detected in these organelles. However, virus particle aggregates were frequently associated with the outer membrane of chloroplasts and occasionally with peroxisomes. Chloroplasts were purified by Percoll gradient, and the coat protein and virus-associated RNAs were extracted and analyzed by Western and Northern blots respectively. Coat protein and CoLV-associated RNAs were not detected within this organelle. The results presented in this work indicate that the association CoLV/chloroplasts, observed in the ultrastructural studies, might be a casual event in the host cell, and that the virus does not replicate inside the organelle. UNESP IBILCE Departamento de Zoologia e Botânica UNESP IBILCE Departamento de Zoologia e Botânica
Databáze:	OpenAIRE
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