Clinical and Molecular Microbiological Evaluation of Regenerative Endodontic Procedures in Immature Permanent Teeth
| Autor: | Laura C.L. Nardello, Ana Carolina C.L. Cerqueira-Neto, Ericka Tavares Pinheiro, Juliana Yuri Nagata, Elizabeth Ferreira Martinez, Marcos Frozoni, Brenda Paula Figueiredo de Almeida Gomes, Adriana de-Jesus-Soares, Marina Carvalho Prado, Andrea Cardoso Pereira |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Regenerative Endodontics Sodium Hypochlorite Root canal Dentistry Calcium Hydroxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Molecular microbiology Dental Pulp Necrosis medicine General Dentistry Permanent teeth Calcium hydroxide Root Canal Irrigants ENDODONTIC PROCEDURES biology business.industry Chlorhexidine 030206 dentistry biology.organism_classification Root Canal Therapy 030104 developmental biology medicine.anatomical_structure chemistry Sodium hypochlorite Dental Pulp Cavity business Periapical Periodontitis Root Canal Preparation Bacteria medicine.drug |
| Zdroj: | Journal of Endodontics. 46:1448-1454 |
| ISSN: | 0099-2399 |
| Popis: | Introduction This study evaluated the bacterial levels after regenerative endodontic procedures and their correlation with the treatment outcome using molecular microbiology methods. Methods Root canal samples of 15 necrotic immature teeth were analyzed by quantitative polymerase chain reaction. Bacteria were counted before treatment (S1), after irrigation with 6% sodium hypochlorite (S2), and after intracanal dressing (S3) using either triple antibiotic paste (n = 7) or calcium hydroxide with chlorhexidine (n = 8). The Wilcoxon test for related samples and the Mann-Whitney test were used for statistical analysis (P Results All S1 and S2 samples were positive for bacteria, but the number of positive S3 samples decreased to 53.3% (P = .001). Overall, there was a significant reduction of bacterial levels after each treatment step (S1–S2, P = .001; S2–S3, P = .02). In the triple antibiotic paste and chlorhexidine groups, 57.1% and 50% of S3 samples were positive with median numbers of 6.97 × 103 and 3.59 × 104 bacterial cells, respectively. No significant differences were found between the groups. Periapical healing occurred in all cases despite the presence of low levels of residual bacteria. However, the latter had a negative impact on the thickness of dentinal walls (R2 = 0.0043). Conclusions Although the bacterial levels were drastically reduced after the regenerative endodontic procedures, the residual bacteria influenced the thickness of the dentinal walls. |
| Databáze: | OpenAIRE |
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