Preparation of antibodies against a novel immunosuppressant, FTY720, and development of an enzyme immunoassay for FTY720
| Autor: | Takeyuki Kohno, Norimasa Matsumoto, Wasako Kurio, Shoji Yamaguchi, Haruko Kusumoto, Takaaki Shimizu, Kazuhito Watabe, Kazuo Yanada, Shuji Uchida, Tetsuro Fujita, Ryoji Hirose |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Ovalbumin
Clinical Biochemistry Administration Oral Pharmaceutical Science Biochemistry Antibodies Antigen-Antibody Reactions Immunoenzyme Techniques Structure-Activity Relationship chemistry.chemical_compound Pharmacokinetics Sphingosine Oral administration Myriocin Drug Discovery medicine Animals Molecular Biology chemistry.chemical_classification Chromatography Molecular Structure biology medicine.diagnostic_test Fingolimod Hydrochloride Chemistry Organic Chemistry Stereoisomerism Enzyme Propylene Glycols Immunoassay biology.protein Molecular Medicine Female Rabbits Antibody Haptens Hapten |
| Zdroj: | Bioorganic & Medicinal Chemistry. 14:4182-4192 |
| ISSN: | 0968-0896 |
| DOI: | 10.1016/j.bmc.2006.01.071 |
| Popis: | FTY720 ( 1 ) is a novel immunosuppressant (immunomodulator), derived from ISP-I ( 2 : myriocin and thermozymocidin). To clarify the pharmacokinetic properties of 1 , antibodies against 1 were prepared and a competitive enzyme immunoassay (EIA) was developed. Two kinds of haptens, 3 and 4 , for 1 were synthesized and coupled to ovalbumin (OVA). Rabbits were immunized with 3 -OVA or 4 -OVA, and corresponding antibodies were obtained. Both antibodies recognized the 2-amino-2-(2-phenylethyl)propane-1,3-diol moiety in 1 . Using the anti- 3 -OVA antibody, a competitive EIA for 1 was developed and evaluated. The range of quantification by the EIA was 0.06–10 ng/mL. The application of the EIA has enabled us to measure the FTY720 concentration in serum after oral administration of 1 (1 mg/kg) to rats. |
| Databáze: | OpenAIRE |
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