WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva

Autor: Monica P. Goldklang, Merly C. Vogt, David T. Ng, Stylianos Kosmidis, Felix Fiederling, Hani J Shayya, Urvashi V Thopte, Ana Pinharanda, Armen G. Enikolopov, Ashok Litwin-Kumar, Ines Rodrigues-Vaz, Tanya Tabachnik, Enrico Cannavò, Jeanine D'Armiento, Kyle Stearns, Peter Andolfatto, Barbara Noro, Andres Bendesky, Darcy S. Peterka
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Saliva
Viral Diseases
Physiology
Coronaviruses
viruses
Medical Conditions
Filter Paper
Medicine and Health Sciences
Medicine
Pathology and laboratory medicine
Virus Testing
Vaccines
Multidisciplinary
Viral Vaccine
Home use
Medical microbiology
Body Fluids
Laboratory Equipment
Infectious Diseases
Bioassays and Physiological Analysis
COVID-19 Nucleic Acid Testing
Viruses
RNA
Viral
Engineering and Technology
Viral spread
Anatomy
SARS CoV 2
Pathogens
Research Article
2019-20 coronavirus outbreak
Isolation (health care)
SARS coronavirus
Infectious Disease Control
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Point-of-Care Systems
Science
Equipment
Sensitivity and Specificity
Microbiology
Extraction techniques
Diagnostic Medicine
Virology
Humans
Viral rna
Epidemics
Colorimetric Assays
business.industry
SARS-CoV-2
fungi
Organisms
Viral pathogens
COVID-19
Reproducibility of Results
Biology and Life Sciences
Covid 19
Viral Vaccines
RNA extraction
Highly sensitive
Microbial pathogens
Research and analysis methods
business
Biochemical Analysis
Zdroj: PLoS ONE, Vol 16, Iss 9, p e0257464 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Despite the development of effective vaccines against SARS-CoV-2, epidemiological control of the virus is still challenging due to slow vaccine rollouts, incomplete vaccine protection to current and emerging variants, and unwillingness to get vaccinated. Therefore, frequent testing of individuals to identify early SARS-CoV-2 infections, contact-tracing and isolation strategies remain crucial to mitigate viral spread. Here, we describe WHotLAMP, a rapid molecular test to detect SARS-CoV-2 in saliva. WHotLAMP is simple to use, highly sensitive (3.6 viral RNA copies per microliter of saliva) and specific, as well as inexpensive, making it ideal for frequent screening. Moreover, WHotLAMP does not require toxic chemicals or specialized equipment and thus can be performed in point-of-care settings, and may also be adapted for resource-limited environments or home use. While applied here to SARS-CoV-2, WHotLAMP can be modified to detect other pathogens, making it adaptable for other diagnostic assays, including for use in future outbreaks.
Databáze: OpenAIRE
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