Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays
| Autor: | Wei Wang, Astrid Vabret, Vincent Deubel, Huajie Yan, Lili Hou, Peijun Ren, Jun Sheng, Philippe Buchy, Sek Mardy, Jing Zhang, François Freymuth |
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| Přispěvatelé: | Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Réseau International des Instituts Pasteur (RIIP), Shanghai Nanxiang Hospital, Pediatric Department, China, Institut Pasteur du Cambodge, Laboratoire de Virologie Humaine et Moléculaire [Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN) |
| Rok vydání: | 2009 |
| Předmět: |
viruses
MESH: Flow Cytometry Respiratory virus medicine.disease_cause RNA Virus Infections 0302 clinical medicine Nasopharynx MESH: Child MESH: Reverse Transcriptase Polymerase Chain Reaction Diagnosis Multiplex 030212 general & internal medicine Child Respiratory Tract Infections 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction Respiratory infection Flow Cytometry MESH: Infant 3. Good health [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Child Preschool MESH: RNA Viral Viruses RNA Viral Rhinovirus MESH: RNA Viruses Human coronavirus NL63 Sensitivity and Specificity Article Virus MESH: Viruses 03 medical and health sciences Human metapneumovirus Virology Multiplex polymerase chain reaction medicine Humans RNA Viruses Multiplex RT-PCR MESH: Humans MESH: RNA Virus Infections 030306 microbiology MESH: Child Preschool Infant biology.organism_classification MESH: Sensitivity and Specificity MESH: Nasopharynx MESH: Respiratory Tract Infections |
| Zdroj: | Journal of Virological Methods Journal of Virological Methods, Elsevier, 2009, 162 (1-2), pp.40-5. ⟨10.1016/j.jviromet.2009.07.004⟩ |
| ISSN: | 0166-0934 |
| Popis: | International audience; A 4-tube multiplex RT-PCR (mRT-PCR), which showed higher sensitivity over conventional methods, was previously developed for the diagnosis of 14 viral pathogens of the respiratory tract. Herein the mRT-PCR was compared to the commercial Luminex mPCR-microsphere flow cytometry assay (Resplex II) which allows the detection of 12 different viruses. Eleven different viruses were identified in 91 nasopharyngeal swabs of children with acute respiratory infection, influenza A (IAV) and B, respiratory syncytial virus (RSV), human rhinovirus (hRhV), human echovirus, parainfluenza viruses (PIV) 1, 2, 3 and 4, human metapneumovirus (hMPV), and human coronavirus NL63. The results of the two techniques showed 53 and 40 positive patients by the Resplex II assay and mRT-PCR, respectively, with a concordance in 35 positive and 33 negative patients (74.7%). Individual RT-PCR tests were performed to control viruses not simultaneously detected by the two multiplex assays. The major virus misdiagnosed by mRT-PCR was IAV whereas the major viruses misdiagnosed by Resplex II were PIV1, 3 and 4. The mRT-PCR remains a simple, rapid, and specific assay for the specific detection of respiratory viruses, and can be easily implemented with standards in clinical laboratories at a low cost. |
| Databáze: | OpenAIRE |
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