Isolation of Encephalitozoon cuniculi Using Primary Tissue Culture Techniques from a Rabbit in a Colony Showing Encephalitozoonosis
| Autor: | Yuji Nakaoka, Daisuke Fukui, Gen Bando, Masao Kosuge, Masanori Yamaguchi, Koji Furuya |
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| Rok vydání: | 2001 |
| Předmět: |
Molecular Sequence Data
Biology Kidney Polymerase Chain Reaction law.invention Microbiology Tissue culture law Culture Techniques parasitic diseases Animals Encephalitozoon cuniculi Polymerase chain reaction Base Sequence General Veterinary fungi Sequence Analysis DNA DNA Protozoan Isolation (microbiology) biology.organism_classification Spore Encephalitozoonosis Encephalitozoon Rabbits Primer (molecular biology) Sequence Alignment |
| Zdroj: | Journal of Veterinary Medical Science. 63:203-206 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.63.203 |
| Popis: | Encephalitozoon spores were isolated in a primary tissue culture of the kidneys from an encephalitozoonosis-suspected rabbit in a municipal zoo in Hokkaido. The isolated spores were morphologically characteristic of microsporidial ones in chromotrope stain, and proven to be E. cuniculi by a polymerase chain reaction (PCR) with a species-specific primer set and by direct DNA sequencing of the PCR products. |
| Databáze: | OpenAIRE |
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