Production of osteoclast-activating factor by normal human peripheral blood rosetting and nonrosetting lymphocytes
| Autor: | C. Trummel, John E. Horton, Joost J. Oppenheim, Priscilla Chen, J. J. Baker |
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| Rok vydání: | 1976 |
| Předmět: |
Immunology
Osteoclasts Stimulation Biology Lymphocyte Activation Peripheral blood mononuclear cell Monocytes Bone resorption Rosette (botany) Tissue culture chemistry.chemical_compound Mediator Lectins Humans Immunology and Allergy Lymphocytes Bone Resorption Lymphokines Macrophages DNA Molecular biology chemistry IL1A Immunologic Techniques Thymidine |
| Zdroj: | European Journal of Immunology. 6:732-736 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | This study has tested the ability of human mononuclear cells and the subpopulations of lymphocytes that rosette (E-RFC) or do not rosette (non-E-RFC) with sheep red blood cells to synthesize DNA and to produce a mediator with bone resorbing activity (osteoclast-activating factor, OAF) in response to stimulation with phytohemagglutinin (PHA). Mononuclear cells were stimulated by PHA both to synthesize DNA and to produce a factor that caused bone resorption. E-RFC enriched for T lymphocytes and depleted of macrophages synthesized considerable DNA in response to stimulation with PHA, but were unable to produce significant bone resorbing activity in tissue culture unless macrophages were re-added to the E-RFC. In contrast, mononuclear cells depleted of E-RFC (non-E-RFC enriched for B cells) did not synthesize DNA in response to stimulation with PHA, but did produce a mediator that caused significant bone resorption in tissue culture. In most of the experiments, unstimulated non-E-RFC produced as much OAF as the PHA-stimulated non-E-RFC. These results suggest that both activated T lymphocytes (E-RFC) and B lymphocytes (non-E- RFC) can produce OAF. |
| Databáze: | OpenAIRE |
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