New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges
| Autor: | Miroslava Blechová, Lenka Maletínská, Lenka Žáková, Zuzana Demianová, Blanka Železná, Veronika Nagelová |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Nociception Cart Physiology Stereochemistry Norleucine Neuropeptide Nerve Tissue Proteins Peptide Binding Competitive PC12 Cells Biochemistry Eating Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Appetite Depressants mental disorders medicine Animals Receptor chemistry.chemical_classification Methionine Chemistry Biological activity Peptide Fragments Rats Mice Inbred C57BL nervous system Mechanism of action Cystine medicine.symptom Locomotion hormones hormone substitutes and hormone antagonists |
| Zdroj: | Peptides. 39:138-144 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2012.09.033 |
| Popis: | The CART (cocaine- and amphetamine-regulated transcript) peptide is an anorexigenic neuropeptide that acts in the hypothalamus. The receptor and the mechanism of action of this peptide are still unknown. In our previous study, we showed that the CART peptide binds specifically to PC12 rat pheochromocytoma cells in both the native and differentiated into neuronal phenotype. Two biologically active forms, CART(55-102) and CART(61-102), with equal biological activity, contain three disulfide bridges. To clarify the importance of each of these disulfide bridges in maintaining the biological activity of CART(61-102), an Ala scan at particular S-S bridges forming cysteines was performed, and analogs with only one or two disulfide bridges were synthesized. In this study, a stabilized CART(61-102) analog with norleucine instead of methionine at position 67 was also prepared and was found to bind to PC12 cells with an anorexigenic potency similar to that of CART(61-102). The binding study revealed that out of all analogs tested, [Ala(68,86)]CART(61-102), which contains two disulfide bridges (positions 74-94 and 88-101), preserved a high affinity to both native PC12 cells and those that had been differentiated into neurons. In food intake and behavioral tests with mice after intracerebroventricular administration, this analog showed strong and long-lasting anorexigenic potency. Therefore, the disulfide bridge between cysteines 68 and 86 in CART(61-102) can be omitted without a loss of biological activity, but the preservation of two other disulfide bridges and the full-length peptide are essential for biological activity. |
| Databáze: | OpenAIRE |
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