Ascorbate and Glutamate Release in the Rat Hippocampus After Perforant Path Stimulation: A 'Dialysis Electrode' Study
| Autor: | Simon Shorvon, M. L. Errington, Matthew C. Walker, John G. R. Jefferys, P. T. Galley |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Microdialysis Glutamic Acid Hippocampus Ascorbic Acid Biology Biochemistry Rats Sprague-Dawley Cellular and Molecular Neuroscience Epilepsy chemistry.chemical_compound Neural Pathways Electrochemistry medicine Animals Neurotransmitter Perforant Pathway Glutamate receptor Equipment Design Perforant path medicine.disease Ascorbic acid Electric Stimulation Rats medicine.anatomical_structure chemistry Calibration Neuroscience |
| Zdroj: | Journal of Neurochemistry. 65:725-731 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1995.65020725.x |
| Popis: | Excitatory amino acids have been proposed to play a critical role in the development and maintenance of epileptic seizures and in the development of neuronal damage. Previous animal studies of glutamate during seizures, however, have often failed to measure any rise in glutamate. We have overcome many of the problems of these studies by using an animal model in which epileptic afterdischarges are induced by stimulation of the perforant path, and glutamate and ascorbate are measured using a newly developed microdialysis electrode that combines the advantages of microdialysis and in vivo electrochemistry. We have successfully shown (1) a rise in glutamate after an epileptic afterdischarge, (2) a concomitant initial fall and then a later rise in ascorbate, and (3) progressive dwindling of this effect when afterdischarges are repeated within minutes, despite similar electroencephalographic responses. The possible mechanisms of these effects are discussed and include ascorbate/glutamate heteroexchange, reversal of the glutamate uptake mechanism, and augmentation of glutamate uptake after a seizure. |
| Databáze: | OpenAIRE |
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