Streptococcal group B integrative and mobilizable element IMESag- rpsI encodes a functional relaxase involved in its transfer

Autor: Fabián Lorenzo-Díaz, Carlos Flores, Manuel Espinosa, Pierre-Emmanuel Douarre, Philippe Glaser, Cris Fernández-López, Adrian Baez-Ortega
Přispěvatelé: Universidad de La Laguna [Tenerife - SP] (ULL), Hospital Universitario N.S. de Candelaria [Santa Cruz de Tenerife, Spain], Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Ecologie et Evolution de la Résistance aux Antibiotiques / Ecology and Evolution of Antibiotics Resistance (EERA), Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Department of Veterinary Medicine, Disease Dynamics Unit [UK], University of Cambridge [UK] (CAM), Ciber Enfermedades Respiratorias (Ciberes), Instituto de Salud Carlos III [Madrid] (ISC), Research financed by the Spanish Ministry of Economy and Competitiveness (CSD2008-00013, and BIO2013-49148-C2-2-R to M.E.), Instituto de Salud Carlos III (FIS PI14/00844 to C.F.) and EU (Regional Development Funds, ‘A way of making Europe’, FP7-REGPOT-2012-CT2012-31637-IMBRAIN to C.F.), Sara Borrell contract CD13/00304 to F.L.-D., and the LabEx IBEID (P.G.). C.F.-L. was the recipient of an EMBO Short Term Fellowship (EMBO ASTF 242-2014) at the P.G. laboratory., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 316137,EC:FP7:REGPOT,FP7-REGPOT-2012-2013-1,IMBRAIN(2012), Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, EMBO, Institut Pasteur [Paris]-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
MESH: Sequence Analysis
DNA
Relaxases
integrative and mobilizable elements mobilization
[SDV]Life Sciences [q-bio]
Relaxase
medicine.disease_cause
Genome
Plasmid
MESH: Genetic Variation
Cloning
Molecular
lcsh:QH301-705.5
MESH: Bacterial Proteins
Genetics
Ribosomal Protein S9
General Neuroscience
MESH: DNA Nucleotidyltransferases
Horizontal gene transfer
Chromosomes
Bacterial
MESH: Ribosomal Proteins
3. Good health
relaxases
DNA Nucleotidyltransferases
genomics/microbiology conjugation
horizontal gene transfer
Research Article
conjugation
Ribosomal Proteins
MESH: Interspersed Repetitive Sequences
MESH: Chromosomes
Bacterial
Gene Transfer
Horizontal
Immunology
Biology
General Biochemistry
Genetics and Molecular Biology
Streptococcus agalactiae
03 medical and health sciences
Open Reading Frames
Bacterial Proteins
medicine
MESH: Cloning
Molecular
Gene
Conjugation
Circular bacterial chromosome
Research
Genetic Variation
Streptococcus
Sequence Analysis
DNA
MESH: Open Reading Frames
MESH: Streptococcus agalactiae
MESH: Gene Transfer
Horizontal
Interspersed Repetitive Sequences
030104 developmental biology
lcsh:Biology (General)
Mobile genetic elements
Integrative and mobilizable elements mobilization
Zdroj: Open Biology
Open Biology, 2016, 6 (10), pp.160084. ⟨10.1098/rsob.160084⟩
Open Biology, Vol 6, Iss 10 (2016)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Open Biology, Royal Society, 2016, 6 (10), pp.160084. ⟨10.1098/rsob.160084⟩
ISSN: 2046-2441
DOI: 10.1098/rsob.160084⟩
Popis: 12p.-6 fig.-3 tab.
Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. Although IMEs seem to be the most prevalent class of elements transferable by conjugation, they are poorly known. Here, we have studied a GBS-IME, termed IMESag-rpsI, which is widely distributed in GBS despite not carrying any apparent virulence trait. Analyses of 240 whole genomes showed that IMESag-rpsI is present in approximately 47% of the genomes, has a roughly constant size (approx. 9 kb) and is always integrated at a single location, the 30-end of the gene encoding the ribosomal protein S9 (rpsI). Based on their genetic variation, several IMESag-rpsI types were defined (A–J) and classified in clonal complexes (CCs). CC1 was the most populated by IMESag-rpsI (more than 95%), mostly of type-A (71%). One CC1 strain (S. agalactiae HRC) was deep-sequenced to understand the rationale underlying type-A IMESag-rpsI enrichment in GBS. Thirteen open reading frames were identified, one of them encoding a protein (MobSag) belonging to the broadly distributed family of relaxases MOBV1. Protein MobSag was purified and, by a newly developed method, shown to cleave DNA at a specific dinucleotide. The S. agalactiae HRC-IMESag-rpsI is able to excise from the chromosome, as shown by the presence of circular intermediates, and it harbours a fully functional mobilization module. Further, the mobSag gene encoded by this mobile element is able to promote plasmid transfer among pneumococcal strains,suggesting that MobSag facilitates the spread of IMESag-rpsI and that this spread would explain the presence of the same IMESag-rpsI type in GBS strains belonging to different CCs.
Research financed by the Spanish Ministry of Economy and Competitiveness (CSD2008-00013, and BIO2013-49148-C2-2-R to M.E.), Instituto de Salud Carlos III (FIS PI14/00844 to C.F.) and EU (Regional Development Funds, ‘A way of making Europe’, FP7- REGPOT-2012-CT2012-31637-IMBRAIN to C.F.), Sara Borrell contract CD13/00304 to F.L.-D., and the LabEx IBEID (P.G.). C.F.-L. was the recipient of an EMBO Short Term Fellowship (EMBO ASTF 242-2014) at the P.G. laboratory.
Databáze: OpenAIRE
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