High levels of soluble CD40 ligand and matrix metalloproteinase-9 in serum are associated with favorable clinical evolution in human visceral leishmaniasis
| Autor: | Carla Vanessa Oliveira Silva, Rodrigo Oliveira Passos, Jeffrey A. Guderian, Tatiana Rodrigues de Moura, Fabrícia Alvisi de Oliveira, Malcolm S. Duthie, Steven G. Reed, Roque Pacheco de Almeida, Nayra P. Damascena, Amélia Ribeiro de Jesus, Ajay Bhatia |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Spleen Parasite load Parasite Load Medical microbiology medicine Humans CD40 Antigens Child Prospective cohort study Visceral leishmaniasis CD40 biology Clinical outcome business.industry Matrix metalloproteinase-9 Leishmania chagasi Prognosis medicine.disease Soluble CD40 ligand Infectious Diseases medicine.anatomical_structure Matrix Metalloproteinase 9 Parasitology Immunology biology.protein Leishmaniasis Visceral Female business Biomarkers Research Article |
| Zdroj: | BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| Popis: | Background Soluble CD40 ligand (sCD40L) and matrix metalloproteinase 9 (MMP-9) are inflammation markers and have been poorly described in infectious disease. In this prospective study, we describe the sera kinetics of these two molecules in the course of treatment follow up in human visceral leishmaniasis (VL). Methods Sera from VL patients were collected before and during follow up of regular Antimony treatment. sCD40L and MMP-9 were measured by Luminex assay. Paired analysis by Wilcoxon signed test was used for comparison of values of the same subjects before and after initiation of treatment. Correlations between clinical data and parasite load with the serum levels of sCD40L and MMP-9 were performed by Spearman test. Tests were considered statistically significant if the probability of a type I error was less than 5% (p-value Results While sCD40L and MMP-9 were not observed in sera from non endemic controls which are at low risk of Leishmania chagasi infection, elevated levels were observed in sera from VL patients, and an increase in sCD40L and MMP-9 levels were detectable during the follow-up of VL patients undergoing antimony treatment. sCD40L levels were also high in individuals living in endemic settings at high risk of infection (endemic controls). Additionally, negative correlations were found between spleen sizes and MMP-9 before treatment and sCD40L at day 15 of treatment. Negative correlations were also found between parasite load with both sCD40L and MMP-9. Conclusion Serum sCD40L and MMP-9 are identified as new and simple biomarkers in two situations: (i) monitoring the success of therapy and (ii) predicting favorable clinical outcome of human VL. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink