Chronic exposure to short-chain fatty acids modulates transport and metabolism of microbiome-derived phenolics in human intestinal cells
| Autor: | László Abrankó, Asimina Kerimi, Gary Williamson, Charlotte Grootaert, John Van Camp, Sarka Tumova, Evelien Van Rymenant |
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| Rok vydání: | 2016 |
| Předmět: |
HUMAN COLON
0301 basic medicine SA Salicylic acid Endocrinology Diabetes and Metabolism Metabolite Clinical Biochemistry Muscle Proteins Acetates Biochemistry HESPERETIN HYDROXYCINNAMIC ACIDS chemistry.chemical_compound Intestinal mucosa ATP Binding Cassette Transporter Subfamily G Member 2 Glucuronosyltransferase Intestinal Mucosa ABCG2 ATP-binding cassette sub-family G member 2 (BCRP) SULT Sulfotransferase GENE-EXPRESSION chemistry.chemical_classification Nutrition and Dietetics Symporters Hesperetin Neoplasm Proteins Butyrates FA Ferulic acid Lactates Glucuronide Monocarboxylic Acid Transporters Polyphenol SCFA Short-chain fatty acids Coumaric Acids Phase II metabolism PHASE-II METABOLISM HT-3′-sul Hesperetin-3′-O-sulfate Butyrate Transporter Article DMCA 3 4-dimethoxycinnamic acid HT-3′-glu Hesperetin-3′-O-glucuronide 03 medical and health sciences Short-chain fatty acids BUTYRATE HT-7-glu Hesperetin-7-O-glucuronide Humans HT-7-sul Hesperetin-7-O-sulfate ABCC ATP-binding cassette sub-family C UDP-GLUCURONOSYLTRANSFERASES Molecular Biology SULFOTRANSFERASES 030109 nutrition & dietetics FA-glu Ferulic acid-4-O-glucuronide HT Hesperetin Hesperidin Biology and Life Sciences Polyphenols Biological Transport Metabolism Fatty Acids Volatile FA-sul Ferulic acid-4-O-sulfate Gastrointestinal Microbiome 030104 developmental biology chemistry Gut metabolism Symporter UGT Uridine diphosphate-glucuronosyl transferase Propionate CACO-2 CELLS DHFA Dihydroferulic acid Caco-2 Cells Propionates FERULIC ACID |
| Zdroj: | The Journal of Nutritional Biochemistry JOURNAL OF NUTRITIONAL BIOCHEMISTRY |
| ISSN: | 1873-4847 0955-2863 |
| Popis: | Dietary fiber-derived short-chain fatty acids (SCFA) and phenolics produced by the gut microbiome have multiple effects on health. We have tested the hypothesis that long-term exposure to physiological concentrations of SCFA can affect the transport and metabolism of (poly)phenols by the intestinal epithelium using the Caco-2 cell model. Metabolites and conjugates of hesperetin (HT) and ferulic acid (FA), gut-derived from dietary hesperidin and chlorogenic acid, respectively, were quantified by LC-MS with authentic standards following transport across differentiated cell monolayers. Changes in metabolite levels were correlated with effects on mRNA and protein expression of key enzymes and transporters. Propionate and butyrate increased both FA transport and rate of appearance of FA glucuronide apically and basolaterally, linked to an induction of MCT1. Propionate was the only SCFA that augmented the rate of formation of basolateral FA sulfate conjugates, possibly via basolateral transporter up-regulation. In addition, propionate enhanced the formation of HT glucuronide conjugates and increased HT sulfate efflux toward the basolateral compartment. Acetate treatment amplified transepithelial transport of FA in the apical to basolateral direction, associated with lower levels of MCT1 protein expression. Metabolism and transport of both HT and FA were curtailed by the organic acid lactate owing to a reduction of UGT1A1 protein levels. Our data indicate a direct interaction between microbiota-derived metabolites of (poly)phenols and SCFA through modulation of transporters and conjugating enzymes and increase our understanding of how dietary fiber, via the microbiome, may affect and enhance uptake of bioactive molecules. |
| Databáze: | OpenAIRE |
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