Comparison of AlphaLISA and RIA assays for measurement of wool cortisol concentrations
| Autor: | Alberto Prandi, Antonella Comin, Tanja Peric, Marta Montillo, Mirco Corazzin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine Lamb 03 medical and health sciences Endocrinology 0302 clinical medicine Animal physiology medicine lcsh:Social sciences (General) Animal species lcsh:Science (General) Biological sciences Glucocorticoids AlphaLISA Alternative methods Multidisciplinary Chromatography Sheep medicine.diagnostic_test Chemistry RIA Radioimmunoassay 030104 developmental biology Wool Immunoassay lcsh:H1-99 030217 neurology & neurosurgery Sheep wool hormones hormone substitutes and hormone antagonists Animal science Research Article lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 10, Pp e05230-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Radioimmunoassay (RIA) methods have always represented a technique of choice for the determination of steroids in biological samples. The Amplified Luminescent Proximity Homogenous Assay-Linked Immunosorbent Assay (AlphaLISA) is now emerging as the new-generation immunoassay technology that does not require washing/separation steps. The aim of this study was to adapt the Perkin-Elmer's AlphaLISA kit for wool cortisol and compare it with a RIA wool cortisol assay. Wool from lambs, 35 at birth (A0) and 54 at two months old (A2), was collected and each extract was evaluated for wool cortisol concentrations (HCC) both by RIA and AlphaLISA immunoassay. The two methods showed good precision, sensitivity and specificity for determining HCC. Both methods were able to detect significant differences between the high and the low HCC assessed in lambs at A0 and A2 (P < 0.01). The HCC assessed with RIA were significantly higher than those assessed with AlphaLISA (P < 0.01). Moreover, the correlation between HCC measured using the AlphaLISA and RIA methods was strong (r = 0.878). The regression analyses show a constant and not proportional error. This could be due to the diversity in the dosage steps and to the diversity of the molecules used in the two methods. Results support the validity of using AlphaLISA as an alternative method to RIA for the quantification of cortisol in sheep wool and considering the performances showed it has a great potential to be further applied as an excellent tool to evaluate HCC in samples derived from animal species. AlphaLISA, Glucocorticoids, Lamb, RIA, Sheep, Animal science, Biological sciences, Endocrinology, Animal physiology, Veterinary medicine |
| Databáze: | OpenAIRE |
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